AAV Antibodies
AAV Antibodies
Exclusive AAV Antibodies for AAV-based projects
PROGEN antibodies are useful for the characterization of different stages of an AAV infection and effective in the analyses of the AAV assembly process. There are two main categories of AAV antibodies:
- AAV capsid protein antibodies that specifically react with denatured AAV capsid proteins and thereby recognize the linear epitopes of the viral capsids.
- AAV particle antibodies that specifically react with intact AAV particles, i.e. empty and full capsids and thereby recognize a conformational epitope of the assembled capsids of certain serotypes.
PROGEN also offers a selection of AAV replicase antibodies.
AAV Antibodies
Yes, PROGEN offers several AAV virus particle specific, neutralizing antibodies against AAV serotypes 1, 2, 4, 5, 6, 8, and 9.
For more information on the neutralizing activity of our antibodies check out the internally collected data and publications for the corresponding serotypes. Just click the button for your specific serotype after following the link.
Yes, PROGEN offers a selection of AAV antibodies in sample sizes. Find a list of antibodies available in sample size of 10 µg (lyophilized) here: AAV Antibodies
PROGEN offers a wide range of AAV antibodies in different quantities and formats (e.g. lyophilized and supernatant). A selection of AAV antibodies are also available in 10µg lyophilized sample sizes. For more information on the different AAV antibodies please see AAV Antibodies.
Anti-Adeno-Associated Virus Capsid Proteins Antibodies
For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:
Anti-Adeno-Associated Virus Particle Antibodies
For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:
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AAV 1
- Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794–1808 (2015).
- McCraw et al. Structureof adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20. Virology (2012) 431:40-9.
- Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
- Huttner et al. Genetic modifications of the adeno-associated virus type 2 capsid reduce the affinity and the neutralizing effects of human serum antibodies. Gene Ther (2003) 10:2139-47.
- Lochrie et al. Mutations on the external surfaces of adeno-associated virus type 2 capsids that affect transduction and neutralization. J Virol (2006) 80:821-34.
- Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794–1808 (2015).
- Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739–7751 (2012).
EM structures of assembled AAV1, 2, 4, 5, 6, and 8 capsids with Fab-fragments are available in the following publications:
- Emmanuel, S. N. et al. Parvovirus Capsid-Antibody Complex Structures Reveal Conservation of Antigenic Epitopes Across the Family. Viral Immunolgoy. 34(1) (2021).
- McCraw, S. M. et al. Structure of adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20. Virology. 432(1-2):40-9 (2012).
- Tseng, Y. et al. Adeno-Associated Virus Serotype 1 (AAV1)- and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J Virol. 89(3):1794-1808 (2015).
- Jose, A. et al. High-Resolution Structural Characterization of a New Adeno-associated Virus Serotype 5 Antibody Epitope toward Engineering Antibody-Resistant Recombinant Gene Delivery Vectors. J Virol. 93(1):e01394-18 (2019).
- Silveria, M. A. et al. The Structure of an AAV5-AAVR Complex at 2.5 Å Resolution: Implications for Cellular Entry and Immune Neutralization of AAV Gene Therapy Vectors. Viruses. 12(11):1326 (2020).
AAV Capsid Antibodies
AAV Capsid Protein Antibodies specifically react with denatured AAV capsid proteins.
AAV Particle Antibodies
AAV Particle Antibodies recognize conformational epitopes of the viral capsid proteins that are only present on fully assembled capsids.
AAV Replicase Antibodies
AAV Replicase Antibodies play an important role in AAV genome replication and packing.