AAV2

dotblot of denaturated and fully assembled capsids

Binding Sites and Reactivity

The A20 and A20R antibodies used for the AAV2 Titration ELISAs (PRATV & PRAAV2R) specifically recognize assembled AAV2 capsid, hence epitopes that result from a specific conformational assembly of the capsid proteins VP1, VP2 and VP3. Several publications cited below describe the analysis of binding sites for the A20 antibody using different techniques. Multiple amino acids have been identified, that are very likely to be part of the binding site, especially those that were identified with more than one method (green boxes).

The amino acids of each binding site are located in different parts of the protein chains and are recognized as the epitope of the antibody only in the assembled capsid where they are in close proximity to each other and in the correct conformation. Note that they do not react with denaturated AAV2-VP.

Neutralizing Activity of the A20 & A20R Antibody

The A20 (Cat. No.61055) and the A20R antibody (Cat. No.610298S) were tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with the AAV2 particles containing a GFP reporter and the indicated concentration of the A20 or A20R antibody, respectively.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the A20 and A20R antibody by decreasing GFP expression with increasing antibody concentration.

Neutr. activity A20 pic Neutr. activity A20R pic

Available Products

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified
Cat. No. 61055
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA, ICC and neutralization assay
  • Reacts with AAV2, AAV2 7m8 and AAV3 intact particles
  • Isotype: IgG3

Variants from €108.00*
€376.00*
anti-AAV2 (intact particle) mouse recombinant, A20R, Biotin Conjugate
Cat. No. 615298
  • Biotin conjugate
  • Recombinant antibody
  • Suitable for dot blot, ELISA, neutralization assay
  • Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
  • Isotype: mouse IgG1

Variants from €108.00*
€235.00*
anti-AAV2 (intact particle) mouse monoclonal, A20, supernatant
Cat. No. 65155
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for ELISA, ICC and neutralization assay
  • Reacts with AAV2, AAV2 7m8 and AAV3 intact particles
  • Isotype: IgG3

Variants from €108.00*
€217.00*
anti-AAV2 (intact particle) mouse recombinant, A20R, lyophilized, purified
Cat. No. 610298
  • Purified, lyophilized
  • Recombinant antibody
  • Suitable for dot blot, ELISA, neutralization assay
  • Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
  • Isotype: mouse IgG1

Variants from €108.00*
€376.00*
anti-AAV2 (intact particle) mouse recombinant, A20R, Biotin Conjugate
Cat. No. 615298
  • Biotin conjugate
  • Recombinant antibody
  • Suitable for dot blot, ELISA, neutralization assay
  • Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
  • Isotype: mouse IgG1

Variants from €108.00*
€235.00*