AAV8

Binding Sites and Reactivity

dotblot serotype crossreactivity pic1

The ADK8 antibody used for the AAV8 Titration ELISA (PRAAV8) specifically recognizes assembled AAV8 capsid, hence epitopes that result from a specific conformational assembly of the capsid proteins VP1, VP2 and VP3.

The publication cited below shows the result of the Cryo-EM analysis of binding sites for the ADK8 antibody. In this publication one potential epitope has been identified which is likely to be part of the ADK8 binding site.

Neutralizing Activity of the ADK8 Antibody

Neutr. Activity pic

The ADK8 antibody (Cat. No. 610160) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV8 particles containing a GFP reporter and the indicated concentration of the ADK8 antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK8 antibody by decreasing GFP expression with increasing antibody concentration.   

  1. Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739–7751 (2012). 
  2. Raupp, C. et al. The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing. J. Virol. 86, 9396–9408 (2012).
  3. Sonntag, F. et al. The assembly activating protein (AAP) promotes capsid assembly of different AAV serotypes. J. Virol. 85, 12686-97 (2011)

Available Products

anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purified
Cat. No. 610160
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization
  • Reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa

Variants from €108.00*
€358.00*
anti-AAV8 (intact particle) mouse monoclonal, ADK8, Biotin Conjugate
Cat. No. 615160
  • Biotin conjugate
  • Mouse monoclonal
  • Suitable for  ELISA
  • Reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa

Variants from €108.00*
€235.00*
anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant
Cat. No. 651160
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization
  • Reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa

Variants from €108.00*
€253.00*
anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9, supernatant
Cat. No. 651161
  • Mouse monoclonal
  • Suitable for dot blot and ICC/IF
  • Reacts with AAV8, AAV9 and AAVrh74 intact particles
  • Isotype: IgG2a kappa

€240.00*