Rapid AAV Titer Determination
In both academia and industry, AAV is the most commonly used viral vector for the delivery of therapeutic transgenes. However, testing individual and crude AAV samples quickly during development and manufacturing is often difficult. Most accurate analytical AAV tools are not convenient for single sample measurements and/or require highly purified samples.
PROGEN's AAV Dip'n'Check lateral flow tests are a quick and easy in-process control for AAV capsid purification processes or for analytical pre-testing. A lateral flow sandwich assay for the semi-quantitative detection of fully assembled AAV capsids using PROGEN's exclusive AAV antibodies.
Available for AAV1, AAV2, AAV3, AAV6, AAV8 and AAV9.
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Your AAV Dip'n'Check Benefits
Reliable in-process control: can be easily implemented both during AAV manufacturing or as a tool to analyze further downstream processes
Fast results: only takes 20 minutes from start to finish
Easy to use: an easy to follow 4-step protocol and easy to use lateral flow test
Suitable for all preparations: can be used with crude lysate or purified AAV material
Capsid-specific: AAV particle antibodies have a binding affinity to the epitopes present only on fully assembled AAV capsids
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What customers are saying
"Dip'n'Check AAV Kits from PROGEN are very easy to use. Semi-quantative readout
by analysing strips with image analysis software tracked extremely well with qPCR-based viral genome titer assays in our hands."
Tomasz Witkos, AstraZeneca
"For rapid confirmation of AAV production, the PROGEN Dip'n'Check Strips are fantastic."
Analytics Team Leader
"These test strips were a perfect way to confirm which PROGEN ELISA kit to use when dealing with serotypes that have variations."
AskBio
AAV Dip'n'Check FAQs
No, the Dip’n’Check assay detects all assembled capsids independent of filling grade.
The Dip’n’Check assay can be used with crude lysate. Please find the concentrations of tested tolerated buffer additives in the manual in section 10. "Matrix Effects" or on the product page in the picture showing Table 1 "Concentrations of tolerated buffer additives".
Possible applications:
- To compare AAV titers from different AAV preparations. With this information you can select the most promising sample for further processing.
- To estimate AAV titers in crude and purified AAV preparations. To either control efficiency of your processes or to pre-test AAV concentrations to hit the dynamic range of downstream analytics.
- To test the binding of common AAV antibodies to an AAV capsid variant. Allowing you to confirm which antibody-based methods are suitable for further analysis.
More information about the determination of the Dip’n’Check can be found on our poster: Potential Applications for AAV Lateral Flow Tests
The lateral flow assay detects 1E+08 to 1E+10 total capsids in the assay volume, which corresponds to a concentration of 1E+09-1E+11 capsids/ml of the starting material.
AAV Dip'n'Check Kits
A quick and easy in-process control for AAV capsid purification processes or analytical pre-testing
AAV Titration ELISA
ELISAs for quantification and detection of full and empty capsids.
AAV Standards
Reliable reference materials to ensure the validity of your results and comparability across assays.
