AAV2 ELISA Control
- Consists of fully assembled, empty AAV2 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
Product description
| Quantity | 1 vial (0.8E+09-1.4E+09 capsids) |
|---|---|
| Application | ELISA |
| Storage | 2-8°C |
| Intended use | Research use only |
| Concentration | 1.7E+09-2.8E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial) |
| Formulation | Lyophilized, empty AAV2 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV2 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer. |
| Stability | 4 weeks at 2-8°C after reconstitution in ASSB 1x |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV2 Titration ELISA) and analysis at least in duplicates is recommended. |
Background
The AAV2 ELISA Control consists of fully assembled, empty AAV2 capsids. The concentration is lot specific.
The AAV2 ELISA Control can be used as a positive control with PROGEN's AAV2 Titration ELISA (PRATV & PRAAV2R). If you require different lots of the AAV2 ELISA Control and the Kit Control included in your PROGEN AAV2 Titration ELISA, please enquire to check availability.
Reading of the AAV2 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN's AAV2 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.
The AAV2 ELISA Control has been calibrated on the ATCC AAV2 reference standard material (RSM, VR-1616). For further information, please see the publication by Lock et al., 2010.
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FAQs
The serotype-specific positive controls are fully characterized empty AAV capsids standardized with PROGEN´s internal gold standards* (AAV1, AAV3, AAV5, AAV6 & AAV9) or the ATCC international gold standard material (AAV2 & AAV8).
Related Products
- Consists of fully assembled, empty AAV2 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
- Fully assembled, empty AAV2 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- ELISA for quantitation of AAV2 capsids
- Detection of full and empty AAV2 capsids
- A20 antibody is used as capture and detection antibody
- ELISA for quantitation of AAV2 capsids
- Detection of full and empty AAV2 capsids
- A20R antibody is used as capture and detection antibody
• Useful for ratio analysis
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
• Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
• Useful in combination with AAV2 VP1 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
• Useful in combination with AAV2 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
- get results in less than 2 hours and increase your daily sample throughput
- easy workflow & process integration
- same accuracy and reproducibility as the standard AAV2 Titration ELISAs
- A20R antibody is used as capture and detection antibody
- Mouse monoclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
- Isotype: IgG2a
- Mouse monoclonal
- Suitable for ICC/IF, IP and WB
- Reacts with AAV2 and AAVDJ
- Isotype: IgG1
- Mouse monoclonal
- Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
- Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
- Isotype: IgG1
- Rabbit polyclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
- Strong affinity for denatured capsid proteins
- Weak affinity for native capsids
- Purified, lyophilized
- Mouse monoclonal
- Suitable for dot blot, ELISA, ICC and neutralization assay
- Reacts with AAV2, AAV2 7m8 and AAV3 intact particles
- Isotype: IgG3
- Purified, lyophilized
- Recombinant antibody
- Suitable for dot blot, ELISA, neutralization assay
- Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
- Isotype: mouse IgG1
- Mouse monoclonal
- Suitable for Affinity Chromatography, ICC/IF and IP
- Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
- Isotype IgG1
- Purified, lyophilized
- Mouse monoclonal
- Suitable for WB
- Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
- Isotype: IgG1
- Mouse monoclonal
- Suitable for WB
- Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
- Isotype: IgG1
- Mouse Monoclonal
- Suitable for WB, ICC/IF and IP
- Reacts with AAV2 Rep52 and Rep78
- Isotype: IgG1
- Rapid lateral flow test for the detection of AAV2 and AAV3 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV2 and AAV3 capsids
- Highly effective for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol
- Automated ELISA kit for quantification of AAV2 virons and assembled capsids
- A20R antibody (Cat. No. 610298) is used as capture and detection antibody
- Suitable for the ProteinSimple Ella system