AAV2 VP1, recombinant protein
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Western blot analysis was performed on different amounts of recombinant AAV2 VP1 ranging from 14 ng to 68 ng. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDye™ 488 Conjugate (Cat. No. 61058-488) was diluted in blocking buffer (antibody concentration 0.13 µg/ml) and incubated for 1 h at RT. The bands were visualized by fluorescent detection.
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10 µl of each solution can be separately loaded onto the SDS PAGE and analyzed by Western blot using the B1 antibody (Cat. No. 690058, Cat. No. 61058-488, Cat. No. 61058-647).
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Undiluted = 100 µg/ml, pre-diluted = 10 µg/ml
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Western blot analysis was performed on different amounts of recombinant AAV2 VP1 ranging from 14 ng to 68 ng. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDye™ 488 Conjugate (Cat. No. 61058-488) was diluted in blocking buffer (antibody concentration 0.13 µg/ml) and incubated for 1 h at RT. The bands were visualized by fluorescent detection.
10 µl of each solution can be separately loaded onto the SDS PAGE and analyzed by Western blot using the B1 antibody (Cat. No. 690058, Cat. No. 61058-488, Cat. No. 61058-647).
Undiluted = 100 µg/ml, pre-diluted = 10 µg/ml
• Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
Product description
| Quantity | 10 µg |
|---|---|
| Application | Capillary electrophoresis (CE), Dot blot, SDS PAGE, WB |
| Purification | Ni-NTA chromatography |
| Storage | -80°C |
| Intended use | Research use only |
| Concentration | 100 µg/ml (1.19 µM) |
| Formulation | Liquid, 6 M urea in PBS |
| Source | Escherichia coli |
| Molecular weight | ~90 kDa |
| Purity | > 90% (determined by SDS PAGE) |
| Product description | N-terminal His-tagged recombinant AAV2 capsid protein VP1 |
| UniprotID | P03135 (AAV2) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Capillary electrophoresis (CE) | Assay dependent |
| Dot Blot | 100 ng, depending on primary antibody and detection method |
| SDS PAGE | 1 µg |
| Western Blot (WB) | 5-20 ng, depending on primary antibody and detection method |
Background
All three recombinant AAV2 capsid proteins are available as set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).
Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
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