- Fully assembled AAV2 capsids filled with an Rluc reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable reagent for cell-based assays (neutralization, transduction)
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
| Filling grade | > 70% full capsids, please find the lot-specific filling grade on the CoA |
|---|---|
| Quantity | 100 µl |
| Application | Cell-based assay, ELISA, Neutralization assay, qPCR |
| Purification | Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific), Iodixanol gradient centrifugation for removal of empty capsids using OptiPrep (Cat. No. 1893, PROGEN) |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Intended use | Research use only |
| Concentration | > 1E+12 vg/ml; please find the lot-specific concentration (total capsid and viral genome titer) on the CoA |
| Formulation | PBS + 0,001% Pluronic F68 |
| Source | Produced in HEK293 based suspension cell line |
| Purity | > 95% by SDS-PAGE using Stain-Free Technology (Bio-Rad) |
| Quality check | Total capsid titer by AAV2 ELISA kits (PRAAV2R or PRAAV2XP), viral genome titer by qPCR, transduction activity, analysis of filling grade, purity, aggregation and endotoxin testing. |
| Transfer Vector | pAAV-CMV-Rluc-WPRE-hGHpA |
| Packaging Plasmid | pRep2-cap2 + pHelper |
| Endotoxin | < 1.0 EU/ml (detection limit 1.0 EU/ml) |
| Note | Please centrifuge before opening to ensure complete recovery of vial contents; aliquoting and repeated freeze/thaw cycles can lead to a drop in titer |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | As a positive control in ELISA |
| Cell-based Assay | Depending on the experimental setup |
| PCR | As standard or positive control in PCR - concentration depending on experimental setup |
| Neutralization Assay | Depending on the experimental setup |
Background
Downloads
FAQs
| Serotype | Transfer vector | Packaging plasmid | Helper plasmid |
| AAV2 | AAV2-CMV-Rluc-WPRE-hGHpA | pRep2-cap2 | pHelper |
| AAV5 | AAV5-CMV-Rluc-WPRE-hGHpA | pRep2-cap5 | pHelper |
| AAV6 | AAV6-CMV-Rluc-WPRE-hGHpA | pRep2-cap6 | pHelper |
| AAV8 | AAV8-CMV-Rluc-WPRE-hGHpA | pRep2-cap8 | pHelper |
| AAV9 | AAV9-CMV-Rluc-WPRE-hGHpA | pRep2-cap9 | pHelper |
Related Products
- Consists of fully assembled, empty AAV2 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
- Fully assembled, empty AAV2 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAV2 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAV2 capsids filled with an Rluc reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable reagent for cell-based assays (neutralization, transduction)
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- ELISA for quantitation of AAV2 capsids
- Detection of full and empty AAV2 capsids
- A20 antibody is used as capture and detection antibody
- ELISA for quantitation of AAV2 capsids
- Detection of full and empty AAV2 capsids
- A20R antibody is used as capture and detection antibody
• Useful for ratio analysis
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
• Useful in combination with AAV2 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
• Useful in combination with AAV2 VP1 and VP3 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
• Useful in combination with AAV2 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
- get results in less than 2 hours and increase your daily sample throughput
- easy workflow & process integration
- same accuracy and reproducibility as the standard AAV2 Titration ELISAs
- A20R antibody is used as capture and detection antibody
- Mouse monoclonal
- Suitable for ICC/IF, IP and WB
- Reacts with AAV2 and AAVDJ
- Isotype: IgG1
- Mouse monoclonal
- Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
- Reacts with denatured VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
- Isotype: IgG1
- Rabbit polyclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
- Strong affinity for denatured capsid proteins
- Weak affinity for native capsids
- Purified, lyophilized
- Mouse monoclonal
- Suitable for dot blot, ELISA, ICC and neutralization assay
- Reacts with AAV2, AAV2 7m8 and AAV3 intact particles
- Isotype: IgG3
- Mouse monoclonal
- Suitable for Affinity Chromatography, ICC/IF and IP
- Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
- Isotype IgG1
- Purified, lyophilized
- Mouse monoclonal
- Suitable for WB
- Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
- Isotype: IgG1
- Mouse monoclonal
- Suitable for WB
- Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
- Isotype: IgG1
- Mouse Monoclonal
- Suitable for WB, ICC/IF and IP
- Reacts with AAV2 Rep52 and Rep78
- Isotype: IgG1
- Recombinant chimeric (human Fc region)
- Suitable for dot blot, neutralization assay and serology ELISA
- Reacts with AAV2 and AAV3 intact capsids
- Isotype: IgG1
- Human recombinant antibody
- Suitable for dot blot, ELISA and neutralization
- Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAVmyo4A, AAVmyo4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9, AAVDJ, intact particles
- Isotype: Human IgG1
- Also available as mouse chimeric antibody
- Monoclonal recombinant mouse chimeric antibody
- Suitable for dot blot, IF, ELISA and neutralization
- Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAV4A, AAV4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9, AAVDJ intact particles
- Isotype: mouse IgG2a
- Also available as human antibody
- Rapid lateral flow test for the detection of AAV2 and AAV3 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV2 and AAV3 capsids
- Highly effective density gradient media for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol