SDS PAGE with AAV3 empty capsids. The AAV3 VP1, VP2 and VP3 proteins were separated on a 10% SDS PAGE and visualized by Biorad Stain technology. Only VP1, VP2 and VP3 proteins are detectable indicating a purity of the AAV preparation of >95%.
SDS PAGE with AAV3 empty capsids. The AAV3 VP1, VP2 and VP3 proteins were separated on a 10% SDS PAGE and visualized by Biorad Stain technology. Only VP1, VP2 and VP3 proteins are detectable indicating a purity of the AAV preparation of >95%.
- Fully assembled, empty AAV3 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
| Quantity | 100 µl (> 5.0E+11 capsids) |
|---|---|
| Application | Dot blot, ELISA, WB |
| Purification | Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific) |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Intended use | Research use only |
| Concentration | > 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial |
| Formulation | PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl |
| Source | Produced in HEK293T cells |
| Purity | > 95% by SDS-PAGE using Stain-Free Technology (Bio-Rad) |
| Quality check | Final titer was assigned on internal reference material using two AAV3 ELISA kits (PRAAV3R); QC included analysis of filling grade and Endotoxin testing |
| Packaging Plasmid | pRep2-Cap3 + pHelper |
| Product description | Purified empty adeno-associated virus 3 capsids (AAV3) |
| Endotoxin | < 1.0 EU/ml |
| Note | Please centrifuge before opening to ensure complete recovery of vial contents; repeated freeze/thaw cycles and aliquotting can lead to a drop in titer |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Dot Blot | Depending on primary antibody and detection method |
| ELISA | As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV3 ELISA) and analysis at least in duplicates is recommended |
| Western Blot (WB) | Depending on primary antibody and detection method |
Background
The AAV3 empty capsids are provided with titers above 5.0E+12 capsids/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot and ELISA. The lot-specific titers were assigned using two different AAV3 ELISA (PRAAV3R).
Our comprehensive quality control ensures well-characterized capsid material which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay.
PROGEN provides empty capsid material for the AAV serotypes 1, 2, 5, 6, 8, 9, rh10 and rh74.
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- Consists of fully assembled, empty AAV3 capsids
- Represents serotype-specific conformational epitopes
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- Serotype-specific conformational epitopes
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- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
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- Reacts with AAV2 and AAV3 intact capsids
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- Rapid lateral flow test for the detection of AAV2 and AAV3 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV2 and AAV3 capsids
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- Designed for the purification of viruses (such as AAV)
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- Ready-made, sterile and endotoxin-tested solution of iodixanol