AAV5 VP2, recombinant protein

Cat. No. 640834

Available, delivery time 1-10 days

€291.00*

Key Features
•    Recombinant AAV5 capsid protein VP2
•    Useful in combination with AAV5 VP1 and VP3 for ratio analysis (also available as set, Cat. No. 72005)
•    Precise molar concentration
•    Suitable for WB, dot blot, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.


Product description
Quantity 10 µg
Application Dot blot, SDS PAGE, WB
Purification Ni-NTA chromatography
Storage -80°C
Intended use Research use only
Concentration 100 µg/ml (1.45 µM)
Formulation Liquid, 6 M urea in PBS
Source Escherichia coli
Molecular weight ~68 kDa
Purity > 90% (determined by SDS PAGE)
Product description N-terminal His-tagged recombinant AAV5 capsid protein VP2
Applications
Tested applications Tested dilutions
Dot Blot 100 ng, depending on primary antibody and detection method
SDS PAGE 1 µg
Western Blot (WB) 5-20 ng, depending on primary antibody and detection method
Background

The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
The recombinant AAV5 VP2 protein in combination with recombinant AAV5 VP1 (Cat. No. 640833) and recombinant AAV5 VP3 (Cat. No. 640835) can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV5 capsid proteins are available as a set (Cat. No. 72005) or as individual proteins (Cat. No. 640833, 640834, 640835).

Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.

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