AAV5 empty capsids

Cat. No. 66V050

Available, delivery time 1-7 days

€269.00*

Key Features
  • Fully assembled, empty AAV5 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
Quantity 100 µl (> 5.0E+11 capsids)
Application Dot blot, ELISA, WB
Purification Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific)
Storage Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid freeze/thaw cycles
Intended use Research use only
Concentration > 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial
Formulation PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl
Source Produced in HEK293T cells
Purity > 95% by SDS-PAGE and silver stain
Quality check Final titer was assigned on internal reference material using two AAV5 ELISA kits (PRAAV5 and PRAAV5XP); QC included analysis of filling grade by analytical ultra centrifugation (AUC) and Endotoxin testing
Product description Purified empty adeno-associated virus 5 capsids (AAV5)
Endotoxin < 1.0 EU/ml
Vector pDP5rs
Note Please centrifuge before opening to ensure complete recovery of vial contents
Applications
Tested applications Tested dilutions
Dot Blot Depending on primary antibody and detection method
ELISA As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV5 ELISA) and analysis at least in duplicates is recommended
Western Blot (WB) Depending on primary antibody and detection method
Background
Our AAV5 empty capsids consist of fully assembled, empty AAV5 capsids and have a titer of > 5.0E+12 viral particles/ml (vp/ml). Final concentrations are lot-specific and can be found on the corresponding vial.

The AAV5 empty capsids are provided with titers above 5.0E+12 viral particles/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot and ELISA. The lot-specific titers were assigned according to our internally established standard material* using two different AAV5 ELISA (PRAAV5 and PRAAV5XP).
Our comprehensive quality control ensures well-characterized capsid material which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay.

PROGEN also provides empty capsid material for the AAV serotypes 1, 2, 6, 8 and 9.

*Our internal standard material for each serotype was characterized according to the protocol described in our poster „Developing Reliable AAV Standards for ELISA“ (available in the downloads tab). Data on the establishment of standard material for specific serotypes can be found as part of the performance data for the corresponding ELISAs or can be provided upon request.

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