AAV6 VP3, recombinant protein

Cat. No. 640838

Available, delivery time 1-10 days

€291.00*

Key Features
•    Recombinant AAV6 capsid protein VP3
•    Useful in combination with AAV6 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72006)
•    Precise molar concentration
•    Suitable for WB, dot blot, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.



Product description
Quantity 10 µg
Application Dot blot, SDS PAGE, WB
Purification Ni-NTA chromatography
Storage -80°C
Intended use Research use only
Concentration 100 µg/ml (1.61 µM)
Formulation Liquid, 6 M urea in PBS
Source Escherichia coli
Molecular weight ~62 kDa
Purity > 95% (determined by SDS PAGE)
Product description N-terminal His-tagged recombinant AAV6 capsid protein VP3
Applications
Tested applications Tested dilutions
Dot Blot 100 ng, depending on primary antibody and detection method
SDS PAGE 1 µg
Western Blot (WB) 5-20 ng, depending on primary antibody and detection method
Background

The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
The recombinant AAV6 VP3 protein in combination with recombinant AAV6 VP1 (Cat. No. 640836) and recombinant AAV6 VP2 (Cat. No. 640837) can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV6 capsid proteins are available as set (Cat. No. 72006) or as individual proteins (Cat. No. 640836, 640837, 640838).

Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
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