AAV8 empty capsids

Cat. No. 66V080

Available, delivery time 1-7 days

€269.00*

Key Features
  • Fully assembled, empty AAV8 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
Quantity 100 µl (> 5.0E+11 capsids)
Application Dot blot, ELISA, WB
Purification Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific)
Storage Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles
Intended use Research use only
Concentration > 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial
Formulation PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl
Source Produced in HEK293T cells
Purity > 95% by SDS-PAGE and silver stain
Quality check

Final titer was assigned on internal reference material using two AAV8 ELISA kits (PRAAV8 and PRAAV8XP); QC included analysis of filling grade and Endotoxin testing

Packaging Plasmid pRep2-Cap8 + pHelper
Product description Purified empty adeno-associated virus 8 capsids (AAV8)
Endotoxin < 5.0 EU/ml
Note Please centrifuge before opening to ensure complete recovery of vial contents; repeated freeze/thaw cycles and aliquoting can lead to a drop in titer
Applications
Tested applications Tested dilutions
Dot Blot Depending on primary antibody and detection method
ELISA As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV8 ELISA) and analysis at least in duplicates is recommended
Western Blot (WB) Depending on primary antibody and detection method
Background
Our AAV8 empty capsids consist of fully assembled, empty AAV8 capsids and have a titer of > 5.0E+12 capsids/ml. Final concentrations are lot-specific and can be found on the corresponding vial. 

The AAV8 empty capsids are provided with titers above 5.0E+12 capsids/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot and ELISA. The lot-specific titers were assigned using two different AAV8 ELISA (PRAAV8 and PRAAV8XP). Internal standard material calibrated with the ATCC reference standard material for AAV8 (VR-1816) was used for the final titer declaration.
Our comprehensive quality control ensures well-characterized capsid material which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay.

PROGEN also provides empty capsid material for the AAV serotypes 1, 2, 5, 6 and 9.

 

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FAQs

The empty capsids are prepared in the absence of an ITR plasmid containing the AAV vector genome, so that no viral vector DNA can be packaged in the particles. In some rare cases the preparations may contain a very small amount of capsids in which fragments of the host cell genome and the packaging plasmids or other DNA fragments have been packaged.

Empty capsids (AAV1, 2, 5, 6, 8, 9, rh10 and rh74) are provided in liquid format in PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl without protein additives. The titer is greater than 5.0E+12 capsids/ml. Therefore, they can be used in several applications.

ELISA Kit Controls (AAV1, 2, 3, 5, 6, 8, 9, rh10 and rh74) are lyophilized. After reconstitution in ASSB 1x the final solution contains stabilizing protein, phenol red and ASSB 1x buffer. The titer is lot-specific around 7.9E+09-1.3E+10 capsids/ml. This product is mainly suitable for the ELISA.

The total capsid titer of our empty capsids is determined by PROGEN ELISA. 

Analysis of the filling grade is performed by AUC or an orthogonal method such as UV/Vis. 

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