AAV8 empty capsids
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- Fully assembled, empty AAV8 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
| Quantity | 100 µl (> 5.0E+11 capsids) |
|---|---|
| Application | Dot blot, ELISA, WB |
| Purification | Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific) |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Intended use | Research use only |
| Concentration | > 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial |
| Formulation | PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl |
| Source | Produced in HEK293T cells |
| Purity | > 95% by SDS-PAGE and silver stain |
| Quality check | Final titer was assigned on internal reference material using two AAV8 ELISA kits (PRAAV8 and PRAAV8XP); QC included analysis of filling grade and Endotoxin testing |
| Product description | Purified empty adeno-associated virus 8 capsids (AAV8) |
| Endotoxin | < 5.0 EU/ml |
| Vector | pRep2-Cap8 + pHelper |
| Note | Please centrifuge before opening to ensure complete recovery of vial contents; repeated freeze/thaw cycles and aliquoting can lead to a drop in titer |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Dot Blot | Depending on primary antibody and detection method |
| ELISA | As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s AAV8 ELISA) and analysis at least in duplicates is recommended |
| Western Blot (WB) | Depending on primary antibody and detection method |
Background
The AAV8 empty capsids are provided with titers above 5.0E+12 viral particles/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot and ELISA. The lot-specific titers were assigned using two different AAV8 ELISA (PRAAV8 and PRAAV8XP). Internal standard material calibrated with the ATCC reference standard material for AAV8 (VR-1816) was used for the final titer declaration.
Our comprehensive quality control ensures well-characterized capsid material which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay.
PROGEN also provides empty capsid material for the AAV serotypes 1, 2, 5, 6 and 9.
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For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
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