- Fully assembled AAV8 capsids filled with an Rluc reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable reagent for cell-based assays (neutralization, transduction)
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
| Filling grade | > 70% full capsids, please find the lot-specific filling grade on the CoA |
|---|---|
| Quantity | 100 µl |
| Application | Cell-based assay, ELISA, Neutralization assay, qPCR |
| Purification | Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific), Iodixanol gradient centrifugation for removal of empty capsids using OptiPrep (Cat. No. 1893, PROGEN) |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Intended use | Research use only |
| Concentration | > 1E+12 vg/ml; please find the lot-specific concentration (total capsid and viral genome titer) on the CoA |
| Formulation | PBS + 0,001% Pluronic F68 |
| Source | Produced in HEK293 based suspension cell line |
| Purity | > 95% by SDS-PAGE using Stain-Free Technology (Bio-Rad) |
| Quality check | Total capsid titer by AAV8 ELISA kits (PRAAV8 or PRAAV8XP), viral genome titer by qPCR, transduction activity, analysis of filling grade, purity, aggregation and endotoxin testing. |
| Transfer Vector | pAAV-CMV-Rluc-WPRE-hGHpA |
| Packaging Plasmid | pRep2-cap8 + pHelper |
| Endotoxin | < 1.0 EU/ml (detection limit 1.0 EU/ml) |
| Note | Please centrifuge before opening to ensure complete recovery of vial contents; aliquoting and repeated freeze/thaw cycles can lead to a drop in titer |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | As a positive control in ELISA |
| Cell-based Assay | Depending on the experimental setup |
| PCR | As standard or positive control in PCR - concentration depending on experimental setup |
| Neutralization Assay | Depending on the experimental setup |
Background
Downloads
FAQs
| Serotype | Transfer vector | Packaging plasmid | Helper plasmid |
| AAV2 | AAV2-CMV-Rluc-WPRE-hGHpA | pRep2-cap2 | pHelper |
| AAV5 | AAV5-CMV-Rluc-WPRE-hGHpA | pRep2-cap5 | pHelper |
| AAV6 | AAV6-CMV-Rluc-WPRE-hGHpA | pRep2-cap6 | pHelper |
| AAV8 | AAV8-CMV-Rluc-WPRE-hGHpA | pRep2-cap8 | pHelper |
| AAV9 | AAV9-CMV-Rluc-WPRE-hGHpA | pRep2-cap9 | pHelper |
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For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
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For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
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- Serotype-specific conformational epitopes
- Reliable reagent for cell-based assays (neutralization, transduction)
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
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