AAVrh74 ELISA Control

Cat. No. PRAAV74-C

Available, delivery time 1-7 days

€110.00*

Key Features
  • Consists of fully assembled, empty AAVrh74 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA
Product description
Quantity 1 vial (1.3E+09-2.2E+09 capsids)
Application ELISA
Storage 2-8°C
Intended use Research use only
Concentration

2.6E+09-4.4E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial)

Formulation Lyophilized, empty AAVrh74 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV ELISAs), incubate 5 min at RT & mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red & ASSB 1x buffer
Stability 4 weeks at 2-8°C after reconstitution in ASSB 1x
Applications
Tested applications Tested dilutions
ELISA To generate a standard curve two-fold serial dilutions in ASSB 1x are recommended. For use as a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV Titration ELISAs) and analysis at least in duplicates is recommended.
Background

The AAVrh74 ELISA Control consists of fully assembled, empty AAVrh74 capsids. The concentration is lot specific.

The AAVrh74 ELISA Control can be used to generate a standard curve adapting PROGEN's AAVrh10 Titration ELISA (Cat. No. PRAAV10). Two-fold serial dilutions of the AAVrh74 ELISA Control in ASSB 1x buffer (provided with the ELISA kit) result in a typical titration curve. The curve allows the quantitative determination of samples of an unkown particle titer. 
The AAVrh74 ELISA control can also be used as a positive control with PROGEN's AAVrh10 ELISA. 

The AAVrh74 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAVrh74 capsids that has been characterized by qPCR and ddPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids).

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FAQs

Empty capsids (AAV1, 2, 5, 6, 8, 9, rh10 and rh74) are provided in liquid format in PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl without protein additives. The titer is greater than 5.0E+12 capsids/ml. Therefore, they can be used in several applications.

ELISA Kit Controls (AAV1, 2, 3, 5, 6, 8, 9, rh10 and rh74) are lyophilized. After reconstitution in ASSB 1x the final solution contains stabilizing protein, phenol red and ASSB 1x buffer. The titer is lot-specific around 7.9E+09-1.3E+10 capsids/ml. This product is mainly suitable for the ELISA.

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