Speed up your His-tag Protein Detection


The proflo His-tag quick test is a competitive immunochromatographic lateral flow assay which allows quick and easy detection of your histidine-tagged proteins. Thus, it represents a fast method to monitor His-tag proteins in small-scale experiments, during your scale-up procedure as well as during your actual purification process. The semi-quantitative assay is suitable for determination of His-tag proteins from cell lysates as well as purified recombinant protein preparations with a concentration range of 2 - 50 µg/ml (for a 25 kDa protein). 

Applications

  • expression monitoring of recombinant His-tag proteins

  • for small-scale screening
  • during expression
  • during scale up
  • during the purification process

Benefits

  • quick results in only 10 min
  • easy handling 2-step protocol
  • faster than SDS-PAGE and western blot
  • works in small sample volumes
  • robust and reliable, similar to WB & ELISA
  • reproducible data

Test Principle

The proflo His-tag quick test is a competitive immunochromatographic lateral flow assay containing a specific anti-His-tag antibody bound to gold particles (capture antibody complex), immobilized His-tag protein (test line) and a control antibody (control line). After application of the sample, the capture antibody complex flows by capillary forces across the strip membrane. With increasing amount of recombinant His-tagged protein in the sample, the intensity of the test line will decrease whereas the control line is independent of the amount of recombinant His-tagged protein and should always appear with the same intensity.


Potential Results:


  • the capture antibody complexes remain unbound
  • at the test line: capture antibody complexes bind to the immobilized His-tag protein
  • at the control line: excessive capture antibody complexes are captured by the control antibody

Result: no His-tag protein in the tested sample.


  • the capture antibody complexes bind the recombinant His-tag protein in the sample
  • at the test line: unbound capture antibody complexes bind the immobilized His-tag protein
  • at the control line: capture antibody complexes bound to the recombinant His-tag protein are captured by the control antibody

Result: intermediate amount of His-tag protein in the tested sample.


  • the capture antibody complexes bind the recombinant His-tag protein in the sample
  • at the test line: less capture antibody complexes are bound to the immobilized His-tag protein
  • at the control line: capture antibody complexes bound to the recombinant His-tag protein are captured by the control antibody

Result: high amount of His-tag protein in the tested sample.



Know what you need?

Login and order directly
from your account.

Order Now

Need more information?

Visit our support hub for FAQs,
references and downloads.

Visit Support Hub