Key Features
- Recombinant bovine basic Fibroblast Growth Factor (bbFGF)
- Growth factor additive in cell culture media
Product description
| Quantity | 1 mg |
|---|---|
| Purification | Heparin affinity chromatography |
| Storage | Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles) |
| Intended use | Research use only |
| Formulation | Lyophilized from 50 mM ammonium formate, pH 7.5 |
| Source | Bovine recombinant, produced in E. coli |
| Molecular weight | 18 kDa (146 aa) |
| Purity | > 95%; sterile |
| Reconstitution | Reconstitute in 1 ml dist. water, dilute further in PBS as required |
| Application | Characterized growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells |
| Synonym | FGF |
Background
Recombinant bovine basic Fibroblast Growth Factor (bbFGF). Growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells.
Biological activity:
< 1 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells)
< 1 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells)
Biological Test System:
- Cell proliferation assay with BAE cell
- Rabbit cornea in vivo assay