- Rapid lateral flow test for the detection of AAV5 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV5 capsids
Product description
| Quantity | 25 Lateral flow tests |
|---|---|
| Reactivity | AAV5 |
| Storage | Box 1: 2-8°C; Box 2: up to 8 weeks at 2-8°C; long term storage at -20°C |
| Intended use | Research use only |
| Product description | Rapid lateral flow test for the detection of AAV5 viral capsids in cell lysates or purified preparations. |
Background
This lateral flow test is a semi-quantitative assay with a detection range of 1.0E+08-1.0E+10 capsids of AAV5 in the assay volume of 156 µl. Within this range, a 2-fold concentration difference is clearly detectable.
Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives
which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
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FAQs
The lateral flow assay detects 1E+08 to 1E+10 total capsids in the assay volume, which corresponds to a concentration of 1E+09-1E+11 capsids/ml of the starting material.
The Dip’n’Check assay can be used with crude lysate. To find out more about the concentrations of tested tolerated buffer additives take a look in the product manual and go to Section 10 - Matrix Effects or you can also find Table 1 - Concentrations of tolerated buffer additives on the individual product website pages.
No, the Dip’n’Check assay detects all assembled capsids independent of filling grade.
The AAV Dip'n'Check test can be used for a variety of applications:
- To compare AAV titers from different AAV preparations. With this information you can select the most promising sample for further processing.
- To estimate AAV titers in crude and purified AAV preparations. To either control efficiency of your processes or to pre-test AAV concentrations to hit the dynamic range of downstream analytics.
- To test the binding of common AAV antibodies to an AAV capsid variant. Allowing you to confirm which antibody-based methods are suitable for further analysis.
More information about the determination of the Dip’n’Check can be found on our poster: Potential Applications for AAV Lateral Flow Tests
Related Products
- Consists of fully assembled, empty AAV5 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
- Fully assembled, empty AAV5 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAV5 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- ELISA for quantitation of AAV5 capsids
- Detection of full and empty AAV5 capsids
- ADK5a antibody is used as capture and detection antibody
- get results in less than 2 hours and increase your daily sample throughput
- easy workflow & process integration
- same accuracy and reproducibility as the standard AAV5 Titration ELISA
- ADK5a antibody is used as capture and detection antibody
- Mouse monoclonal
- Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
- Reacts with denatured VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
- Isotype: IgG1
- Rabbit polyclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
- Strong affinity for denatured capsid proteins
- Weak affinity for native capsids
- Purified, lyophilized
- Mouse monoclonal
- Suitable for dot blot, ELISA and neutralization assay
- Reacts with AAV5 intact capsids
- Isotype: IgG2a kappa
- Purified, lyophilized
- Mouse monoclonal
- Suitable for ELISA, ICC and neutralization assays
- Reacts with AAV5 intact capsids
- Isotype: IgG2b kappa
- Rabbit polyclonal
- Suitable for Affinity Chromatography and WB
- Reacts with AAV5
- Recombinant chimeric (human Fc region)
- Suitable for dot blot, neutralization assay and serology ELISA
- Reacts with AAV5 intact capsids
- Isotype: IgG1
- Recombinant chimeric (human Fc region)
- Suitable for dot blot, neutralization assay and serology ELISA
- Reacts with AAV5 intact capsids
- Isotype: IgG2
- Rapid lateral flow test for the detection of AAV5 capsids
- Semi-quantitative AAV titer determination in cell lysates and purified preparations
- Detection of full and empty AAV5 capsids
- Highly effective density gradient media for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol