Endonuclease ELISA

Cat. No. PRNUC

Prices excl. VAT

Available, delivery time: 1-7 days

€680.00*

Key Features

ELISA for quantitative in vitro measurement of Serratia marcescens endonucleases
Test may also be used to monitor the removal of endonucleases such as DENARASE^® or Benzonase^® Nuclease from process related samples for process development and quality control purposes

Product description

Quantity	96 tests
Application	ELISA
Reactivity	Serratia marcescens endonuclease
Storage	2-8°C
Intended use	Research use only
Application	ELISA
Form supplied	Reagent kit, 12 x 8-well strips

Background

This ELISA kit is intended for the quantitative in vitro measurement of Serratia marcescens endonuclease. This test may be used to monitor the removal of endonucleases such as DENARASE^® or Benzonase^® Nuclease from process related samples for process development and quality control purposes.

The ELISA principle: This Endonuclease ELISA kit is a sandwich ELISA to be performed in a microtiter plate format. A specific monoclonal capture antibody is coated onto wells of a microtiter plate and is used to capture Serratia marcescens endonuclease from samples. Captured endonucleases are detected in two steps:

1. A biotin-conjugated monoclonal detector antibody is added.

2. A streptavidin peroxidase conjugate reacts with the biotin molecules.

Adding substrate solution results in a colour reaction, which is proportional to the analyse concentration present in the wells. The absorbance is measured photometrically at 450 nm (optional: reference wavelength between 620 – 690 nm). The endonuclease concentration in unknown samples can be calculated based on the corresponding DENARASE^® standard curve.

Downloads

File

Category

Size

Filetype

File Endonuclease Elisa Manual

Category Kit manual

Size 335.67 KB

Filetype pdf

MSDS

FAQs

This Endonuclease ELISA kit is intended for the quantitative in vitro measurement of an endonuclease of Serratia marcescens. This assay can be used to monitor the presence of endonucleases, such as DENARASE®, DENARASE® High Salt, Benzonase®, or EndoCleave, in process-related samples during process development and quality control. The assay includes the enzyme DENARASE® as a standard to generate the standard curve. When measuring different endonucleases with this assay, for accurate quantification, correction factors may be applied or custom enzyme standards may be used.

Endonuclease assay provides ultra-sensitive detection with a limit of detection (LOD) of 4 pg/ml and a limit of quantification (LOQ) of 12 pg/ml — 15–20× more sensitive than comparable kits. The dynamic range is 32–1000 pg/ml.

The Endonuclease kit is ready-to-use with pre-coated plates and reagents included, highly reproducible, broadly applicable across different endonucleases. It supports efficient workflows (up to 50% shorter incubation compared to competitor kits).

The Endonuclease assay is designed for monitoring residual nucleases during AAV and viral vector production, supporting QC in biologics and recombinant protein manufacturing, and ensuring compliance in CDMOs and CROs. It is for research use only.

In the ELISA kit manual a reference wavelength is given. The primary measurement is taken at 450 nm, so your instrument must include a suitable 450 nm filter. While the reference wavelength (between 620 nm and 690 nm) can be used, it is not strictly required. Dual-wavelength readings can, in principle, improve accuracy by correcting for non-specific optical effects in the plate. When available, reader software will automatically subtract the reference absorbance from the 450 nm signal. However, because the plates included in the kit are of high optical quality, subtracting reference values typically does not provide a noticeable statistical advantage.

Endonuclease kits remain stable during shipping and short-term storage at room temperature but should be stored at 2–8 °C upon arrival for optimal preservation. All reagents are stress-tested to ensure stability during transport, even under challenging conditions.

Contact

Need more product information?

Learn more

Related Products

AAV1 empty capsids

Cat. No. 66V010

Fully assembled, empty AAV1 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV2 empty capsids

Cat. No. 66V020

Fully assembled, empty AAV2 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV2 VP1 + VP2 + VP3, recombinant proteins, set

Cat. No. 72001

•    Recombinant AAV2 VP1-3 capsid protein set
•    Useful for ratio analysis
•    Precise molar concentration
•    Suitable for WB, dot blot, CE, SDS PAGE

Not available in China and in the Russian Federation.

For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€753.00*

AAV2 Xpress ELISA

Cat. No. PRAAV2XP

Fast, easy and reliable AAV2 titer determination:

get results in less than 2 hours and increase your daily sample throughput
easy workflow & process integration
same accuracy and reproducibility as the standard AAV2 Titration ELISAs
A20R antibody is used as capture and detection antibody

€685.00*

AAV5 empty capsids

Cat. No. 66V050

Fully assembled, empty AAV5 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV5 Xpress ELISA

Cat. No. PRAAV5XP

Fast, easy and reliable AAV5 titer determination:

get results in less than 2 hours and increase your daily sample throughput
easy workflow & process integration
same accuracy and reproducibility as the standard AAV5 Titration ELISA
ADK5a antibody is used as capture and detection antibody

€685.00*

AAV6 empty capsids

Cat. No. 66V060

Fully assembled, empty AAV6 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV6 Xpress ELISA

Cat. No. PRAAV6XP

Fast, easy and reliable AAV6 titer determination:

get results in less than 2 hours and increase your daily sample throughput
easy workflow & process integration
same accuracy and reproducibility as the standard AAV6 Titration ELISA
ADK6 antibody is used as capture and detection antibody

€685.00*

AAV8 Xpress ELISA

Cat. No. PRAAV8XP

Fast, easy and reliable AAV8 titer determination:

Get results in less than 2 hours and increase your daily sample throughput
Easy workflow & process integration
Same accuracy and reproducibility as the standard AAV8 Titration ELISA
ADK8 antibody is used as capture and detection antibody

€685.00*

AAV9 empty capsids

Cat. No. 66V090

Fully assembled, empty AAV9 capsids
Serotype-specific conformational epitopes
Reliable positive control for dot blot, western blot, ELISA
Quality controlled for purity, AAV titer and filling grade
Alignment to internal reference standard material

Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*

AAV9 Xpress ELISA

Cat. No. PRAAV9XP

Fast, easy and reliable AAV9 titer determination:

get results in less than 2 hours and increase your daily sample throughput
easy workflow & process integration
same accuracy and reproducibility as the standard AAV9 Titration ELISA
ADK9 antibody is used as capture and detection antibody

€685.00*

anti-AAV VP1/VP2 mouse monoclonal, A69

Cat. No. 690057

Quantity: 1 ml (50 µg/ml)

Mouse monoclonal
Suitable for ICC/IF, IP and WB
Reacts with AAV2 and AAVDJ
Isotype: IgG1

€363.00*

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified

Cat. No. 690058

Quantity: 1 ml

Mouse monoclonal
Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
Reacts with denatured VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
Isotype: IgG1

Variants from €110.00*

€382.00*

anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum

Cat. No. 61084

Rabbit polyclonal
Suitable for ELISA, ICC/IF, IP and WB
Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
Strong affinity for denatured capsid proteins
Weak affinity for native capsids

€382.00*

anti-AAV2 Replicase mouse monoclonal, 303.9, lyophilized, purified

Cat. No. 61069

Mouse monoclonal
Suitable for WB
Reacts with AAV2 Rep40, Rep52, Rep68 and Rep78
Isotype: IgG1

€389.00*

anti-AAV2 Replicase mouse monoclonal, 76.3, lyophilized, purified

Cat. No. 61073

Mouse Monoclonal
Suitable for WB, ICC/IF and IP
Reacts with AAV2 Rep52 and Rep78
Isotype: IgG1

€370.00*

anti-pan AAV (intact particle) human recombinant, 1C4H

Cat. No. 692373

Quantity: 1 ml (50 µg/ml)

Human recombinant antibody
Suitable for dot blot, ELISA and neutralization
Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAVmyo4A, AAVmyo4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9, AAVDJ, intact particles
Isotype: Human IgG1
Also available as mouse chimeric antibody

Variants from €110.00*

€345.00*

anti-pan AAV (intact particle) mouse chimeric, 1C4M

Cat. No. 690383

Quantity: 1 ml (50 µg/ml)

Monoclonal recombinant mouse chimeric antibody
Suitable for dot blot, IF, ELISA and neutralization
Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAV4A, AAV4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9, AAVDJ intact particles
Isotype: mouse IgG2a
Also available as human antibody

Variants from €110.00*

€345.00*

Dip’n’Check AAV1 and AAV6 - lateral flow assay

Cat. No. PR5216

Rapid lateral flow test for the detection of AAV1 and AAV6 capsids
Semi-quantitative AAV titer determination in cell lysates and purified preparations
Detection of full and empty AAV1 and AAV6 capsids

€163.00*

Dip’n’Check AAV2 and AAV3 - lateral flow assay

Cat. No. PR5223

Rapid lateral flow test for the detection of AAV2 and AAV3 capsids
Semi-quantitative AAV titer determination in cell lysates and purified preparations
Detection of full and empty AAV2 and AAV3 capsids

€163.00*

Dip’n’Check AAV5 - lateral flow assay

Cat. No. PR5205

Rapid lateral flow test for the detection of AAV5 capsids
Semi-quantitative AAV titer determination in cell lysates and purified preparations
Detection of full and empty AAV5 capsids

€163.00*

Dip’n’Check AAV8 - lateral flow assay

Cat. No. PR5208

Rapid lateral flow test for the detection of AAV8 capsids
Semi-quantitative AAV titer determination in cell lysates and purified preparations
Detection of full and empty AAV8 capsids

€163.00*

Dip’n’Check AAV9 - lateral flow assay

Cat. No. PR5209

Rapid lateral flow test for the detection of AAV9 capsids
Semi-quantitative AAV titer determination in cell lysates and purified preparations
Detection of full and empty AAV9 capsids

€163.00*

OptiPrep™ - Iodixanol

Cat. No. 1893

Highly effective density gradient media for the separation of full and empty AAV particles
Designed for the purification of viruses (such as AAV)
Useful to isolate different cell types and organelles
Ready-made, sterile and endotoxin-tested solution of iodixanol

€178.00*

Endonuclease ELISA

What does the Endonuclease ELISA detect?

How sensitive is the assay?

What are the key benefits of Endonuclease ELISA?

What are typical applications?

What does reference wavelength mean in the manual?

How should the ELISA kit be stored?