Epidermal Growth Factor (hEGF), human recombinant, 500 µg
Verification of hEGF with NHDF in a proliferation assay (Rotitest)
- Recombinant human Epidermal Growth Factor (hEGF)
- Growth factor additive in cell culture media
|Purification||Ion exchange chromatography|
|Storage||Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)|
|Intended use||Research use only|
|Source||Human recombinant, produced in E. coli|
|Molecular weight||6.2 kDa|
|Purity||> 98% (determined by SDS gelelectrophoresis)|
|Reconstitution||Reconstitute in 1 ml dist. water, resulting in a final EGF concentration of 0.5 mg/ml PBS.
Dilute further in PBS as required.
|Application||Characterized growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells|
Recombinant human Epidermal Growth Factor (hEGF). Growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells.
< 15 ng/ml for half maximum stimulation of cell proliferation with NHDF cells.
Biological Test System:
Cell proliferation assay with NHDF cells.
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