Verification of hEGF with NHDF in a proliferation assay (Rotitest)
Verification of hEGF with NHDF in a proliferation assay (Rotitest)
Key Features
- Recombinant human Epidermal Growth Factor (hEGF)
- Growth factor additive in cell culture media
Product description
| Quantity | 500 µg |
|---|---|
| Purification | Ion exchange chromatography |
| Storage | Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles) |
| Intended use | Research use only |
| Source | Human recombinant, produced in E. coli |
| Molecular weight | 6.2 kDa |
| Purity | > 98% (determined by SDS gelelectrophoresis) |
| Reconstitution | Reconstitute in 1 ml dist. water, resulting in a final EGF concentration of 0.5 mg/ml PBS. Dilute further in PBS as required. |
| Application | Characterized growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells |
| UniprotID | |
| Synonym | EGF |
Background
Recombinant human Epidermal Growth Factor (hEGF). Growth factor additive in cell culture media for keratinocytes, epithelial and epidermal cells.
Biological Activity:
< 15 ng/ml for half maximum stimulation of cell proliferation with NHDF cells.
Biological Test System:
Cell proliferation assay with NHDF cells.