Glial Filament Protein, bovine, 250 µg

Cat. No. 62007

Available, delivery time 1-7 days

€306.00*

Key Features
  • Purified bovine glial filament protein (GFP, also designated GFAP)
  • Protein standard
Product description
Quantity 250 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Bovine spinal cord
Molecular weight 52 kDa
Isoeletric point pI 5.4
Purity > 95% (determined by SDS gelelectrophoresis)
Reconstitution
Reconstitute with 200 µl distilled water (final volume 250 µl).
Final solution: 10 mM sodium phosphate buffer pH 7.5, 6 M urea, 2 mM DTT, 1 mM EDTA, 10 mM methylammonium chloride;
Protein concentration: 1 mg/ml
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Synonym GFAP, GFP
Background
Glial Filament Protein (GFAP) standard for immunoblotting, immunization and immunoassays.
Reconstitution to filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M and Franke WW (1985). J Cell Biol 101, 1826-1841
- Hatzfeld M et al. (1987). J Mol Biol 197, 237-255
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