Keratin K14, human recombinant, 100 µg

Cat. No. 62218

Available, delivery time 1-7 days

€300.00*

Key Features
  • Recombinant human keratin K14 (formerly also designated cytokeratin 14)
  • Protein standard
Product description
Quantity 100 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Human recombinant, produced in E. coli
Molecular weight 50 kDa
Isoeletric point pI 5.3
Purity > 95% (determined by SDS gelelectrophoresis)
Reconstitution Reconstitute with 70 µl distilled water (final volume 100 µl).
Final solution: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride;
Protein concentration: 1 mg/ml
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Synonym Cytokeratin 14
Background
Human recombinant Keratin K14 for use in immunoblotting and ELISA.
Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
Downloads
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


Related Products

anti-Keratin K14 guinea pig polyclonal, serum
Cat. No. GP-CK14
Quantity: 100 µl
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse

Variants from €49.00*
€297.00*