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Keratin K8, human recombinant, 100 µg

Cat. No. 62213

Prices excl. VAT

Available, delivery time: 1-7 days

€239.00*

Datasheet
Key Features
  • Recombinant human keratin K8 (formerly also designated cytokeratin 8)
  • Protein standard
Product description
Quantity 100 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Human recombinant, produced in E. coli
Molecular weight 52 kDa
Isoeletric point pI 6.1
Purity > 95% (determined by SDS gelelectrophoresis)
Reconstitution Reconstitute with 70 µl distilled water (final volume 100 µl).
Final solution: 30 mM Tris/HCI pH 8, 9 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride;
Protein concentration: 1 mg/ml
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Synonym Cytokeratin 8
Background
Protein standard for immunoblotting, immunization and immunoassays.
Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
References/Publications (1)
Publication
Species
Application
Publication Hofmann, I. & Franke, W. W. Heterotypic interactions and filament assembly of type I and type II cytokemtins In vitro : viscometry and determlnatlons of relative affinities. 132, 122–132 (1997).
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Datasheet
MSDS
FAQs
Reconstitution to filaments is performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.2-0.5 mg/ml, both dissolved in 9.5 M urea buffer: e.g. cytokeratin 18 solution in urea buffer plus cytokeratin 8 solution in urea buffer (both with the same protein concentration!) Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea buffer  (for approx. 4 h at RT) and then to low salt condition (overnight at 2-8°C, using e.g. 50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). Use a large excess of dialyzing buffer (e.g. x500). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). For storage of the dialyzed solution we recommend division in suitable aliquots and freezing below -50°C. Avoid repeated freezing / thawing cycles.

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