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LNP FluoGreen Protein Delivery Kit

Cat. No. PR6113

Prices excl. VAT

Available, delivery time: 1-10 days

€468.00*

Datasheet
Key Features
  • Easy to use
  • Great protein association efficiency irrespective of the MW and isoelectric point. Suitable also for positively charged proteins
  • High stability in diverse biological media
  • Robust protection against degradation
  • Narrow size distribution
  • High fluorescence and non-toxic cellular uptake, facilitating real-time tracking of protein-loaded nanoparticles in vitro
  • Minimal cytotoxicity in vitro and in vivo studies
  • Fluorescent nanoparticles for precise tracking of protein internalization
Product description
Quantity 1 kit
Storage before reconstitution -20ºC (avoid freeze/thaw cycles)
Storage after reconstitution Up to 60 days at 2-8 ºC
Intended use Research use only
Reconstitution Formulate in Ethanol 96% per protocol.
Application Nanoparticle reagent kit for FluoGreen Protein delivery
Specificity Proteins
Stability

Do not freeze FluoGreen Protein Delivery LNPs.

Do not heat up FluoGreen Protein Delivery LNPs.

 
Synonym

LNP
Background

Fluorescent Nanoparticle reagent kit for intracellular protein delivery

Specifically designed for precision biomedicine and therapeutic evaluation. 

Applications

In vitro

  • Tracking cell internalization
  • FRET studies
  • Intracellular delivery of therapeutic compounds
  • Therapeutic evaluation
  • Protein-protein interactions
  • Protein overexpression

In vivo

  • Biodistribution evaluation
  • Therapeutic evaluation
  • Cell tracking

About the LNP FluoGreen Protein Delivery Kit:

PROGENs FluoGreen Protein Delivery LNPs come as a fluorescently-tagged, dryed lipid emulsion, easy to formulate by bench-top addition of ethanol. This kit is a breakthrough in protein therapeutics and fluorescence imaging. It ensures effective delivery of protein-loaded nanoparticles, pivotal for research in proteomics, targeted therapy, and cellular biology. 

Its strong fluorescence at Ex/Em = 495/503 nm is perfect for visualizing protein interactions in living cells, which is essential in oncology, immunology, and regenerative medicine. The high biocompatibility of the LNP Protein delivery nanoparticles enables an efficient internalization of the protein whilst yielding class-leading cell viabilities. Moreover, the nanoparticles have shown efficacy in rodents and zebrafish models across various administration methods.

LNP FluoGreen Protein Delivery Kit comprises a preparation of lipids carrying a reactive group to conduct click chemistry, where an azido (N3- group) tagged to the protein/s of interest will covalently react with the surface of the lipid nanoparticles. Thus, a modification in the protein/s of interest must be performed to add an  azido (N3- group) prior to the formulation. This modification has no impact on the structure and activity of the protein. All necessary reagents are provided within the kit.

PROGENs LNP FluoGreen Protein Delivery Kit has been validated with proteins that possess different length (from 17.3 KDa to 540 KDa) and pI (from 4.6 to 8.3). Additionally, PROGEN offers customized solutions with alternative fluorescent labels. Please contact PROGEN for a customized solution.

 

Downloads
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Category
Size
Filetype
File LNP FluoGreen Protein Delivery Kit Manual
Category Kit manual
Size 457.24 KB
Filetype pdf
Datasheet
MSDS
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FAQs
If necessary, the formulation can be concentrated by using an Amicon Ultra Centrifugal Filter, SpeedVac or Rotavap in mild conditions (avoid overpassing 35 °C or drying out the samples). Samples can be concentrated up to 4-fold its original volume (i.e., to a final volume 250 µL).
The concentration of the fluorophore is 4 µg/mL in the final formulation.
Diameter size can be measured by Dynamic Light Scattering (DLS) analysis.
Yes, the formulation can be used in vivo (not for use in humans). For specific recommendations or customized solutions, please contact PROGEN.
Our FluoGreen nanoparticles can be used for cell or organ analysis by flow cytometry or confocal microscopy. However, for whole-body imaging, PROGEN can provide customized reagents labeled with fluorophores such as Cy7.5. We also offer customized solutions to adjust fluorophore concentration as needed.
The tagging of the proteins facilitates their association to the lipid nanoparticles by following easy and mild procedures that do not compromise their structure and activity. The LNP Protein Delivery kit lipid nanoparticles and the LNP FluoGreen Protein Delivery kit lipid nanoparticles do not contain cationic lipids.
All you need for the protein modification step is provided in the kit. You do not need specific equipment other that a pipette and a centrifuge.
The protein tagging does not alter the native protein biological function when performed according to the protocol. Please contact PROGEN for advice depending on your specific molecule. PROGEN can provide alternative procedures.
In case your molecule requires a different association procedure, please contact PROGEN for advice. We have expertise in the association of proteins following alternative approaches. Nevertheless, keep in mind that the proposed modification of the LNP Protein Delivery kit lipid nanoparticles and the LNP FluoGreen Protein Delivery kit has proven to be advantageous in terms of loading, stability and functionality to other modifications.
LNP FluoGreen Protein Delivery Kit can indeed lead to an increased in the intracellular content of the associated protein in much simple and easy way in relation to conventional transfection protocols. The reagents can be added when the cell culture media is replaced, or at specific time points. Please, contact PROGEN for further advice.
Currently, we offer the green fluorochrome. However, you can contact PROGEN for a customized formulation where we can incorporate alternative fluorochromes: Cy5, Cy7.5, and other fluorescent dyes.
Green fluorescence remains stable for up to 1 year at -20 ºC prior to formulation. After preparation, it is stable for at least 7 days (maximum tested time) when stored at 4 ºC, protected from light. In live cells, the signal can be tracked for at least 7 days.
No, it does not. The fluorescence arises from molecules that are covalently linked to the lipids, but have no intrinsic effect. The fluorescence does not interfere with the efficacy of the formulation or the activity of the associated drug/biomolecule.
This will depend on the specific properties of each molecule. Please refer to the specific protocol of each reagent. For in vivo delivery, formulation can be adapted under request to reach specific requirements attending to the dose and route of administration. For small drugs, it can range from 1% to 15% (55 μg - 0,83 mg) with respect to the total amount of lipids (5,5 mg) per vial. We recommend starting with 1%, and then increasing the concentration gradually. For proteins and peptides, it depends on the selected kit and on the specific molecule, but it is possible to load at least 150 ug of protein/peptide per vial.
We have tested in healthy mice to get an insight, but detailed biodistribution should be done with relevant models and with encapsulated drugs / molecules as this affects the biodistribution. We have conducted s.c. (subcutaneous) administration using one or more imaging modalities as we have optimized labeling (PET, SPECT and fluorescent tracking), i.v. (tail vein injection and retroorbital), i.a. (intra-articular), and i.p. (intraperitoneal).
Once formulated, we recommend using the formulation within 24-48 h, according to the provided protocol.
It is not necessary for initial in vitro testing. There is not a big increase in viscosity and the pH from the formulation is suitable for in vitro testing. It Is also possible to use buffers to formulate, as indicated in the kit protocol.
Filtration through 0,45 μm or 0,22 μm will work to eliminate free precipitated compound to obtain the percentage of free compound and then calculate the percentage of encapsulation through the subtraction of the total and free concentrations, in relation to the total concentration. The filtered fraction that has passed through the filter should be analyzed.
Cargo can enter the cell by either endocytosis or passive membrane fusion.
Yes. Formulations are monodisperse with a diameter size around 100 nm.
1 mL of Protein Reagent is sufficient to perform 225 delivery experiments in 6-well plates, 1150 experiments in 24-well plates and 6815 experiments in 96-well plates.

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