LNP FluoGreen mRNA Delivery Kit
- Easy to use
- Great mRNA transfection efficiency
- Robust protection against degradation
- Suitable for 2D and 3D cultures
- Stable and homogeneous structure, yielding high cell viability
- Narrow size distribution
- Effective in vivo without cationic lipids in the emulsion
- Minimal cytotoxicity in vitro and in vivo
- High fluorescence and non-toxic cellular uptake, facilitating real-time tracking of mRNA-loaded nanoparticles in vitro.
Product description
Quantity | 1 kit |
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Storage before reconstitution | -20ºC (avoid freeze/thaw cycles) |
Storage after reconstitution | Up to 24 hours at 2-8 ºC |
Intended use | Research use only |
Reconstitution | Formulate in Ethanol 96% and 10 mM Citrate Buffer, pH 3 per protocol. |
Application | Nanoparticle reagent kit for FluoGreen mRNA delivery |
Specificity | mRNA |
Stability | Do not freeze FluoGreen mRNA Delivery LNPs. Do not heat up FluoGreen mRNA Delivery LNPs.
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Synonym | LNP |
Background
Fluorescent nanoparticle reagent kit for mRNA targeted delivery
A breakthrough in gene therapy and molecular biology enabling efficient mRNA transfection in cells, including primary cultures and organoids.
Applications
In vitro:
Delivery and tracking of different types of oligonucleotides for:
- Gene addition or replacement: for example, mRNA encapsulation.
- Gene expression control; e.g. interference studies with siRNA, miRNA, circRNA, and ASOs in living-and fixed cells are possible with customized solutions (available upon request).
- Gene editing: customized solutions suitable for delivery of CRISPR–Cas9 gene editing or base-editing systems (available upon request).
In vivo:
Delivery and tracking of different types of oligonucleotides for:
- Gene addition or replacement: for example, mRNA encapsulation.
- Gene expression control; e.g. interference studies with siRNA, miRNA, circRNA, and ASOs in living-and fixed cells are possible with customized solutions (available upon request).
- Gene editing: customized solutions suitable for delivery of CRISPR–Cas9 gene editing or base-editing systems (available upon request).
Protein expression
Innovative RNA therapeutics and precision medicine research
About the LNP FluoGreen mRNA Delivery Kit:
PROGENs FluoGreen mRNA Delivery LNPs come as a fluorescently-tagged, dryed lipid emulsion, easy to formulate by bench-top reconstitution with ethanol and citrate buffer for adequate mRNA encapsulation. The formulation can be subjected to pH 7 buffer exchange by ultrafiltration. The fluorescence tag is attached to the sphingolipid contained in the formulation (Ex/Em = 495/503 nm). This kit is a key tool for RNA-based therapeutics and cellular imaging: it may be used for delivering different types of oligonucleotides, such as siRNA, miRNA, circRNA, and ASOs.
The high biocompatibility of the LNP FluoGreen mRNA Delivery Kit enables an efficient internalization of the mRNA whilst yielding good cell viabilities in a broad range of mammalian cells, even in difficult-to-transfect cells, including primary cells and organoids. They enable mRNA delivery in living-and fixed cells, which is essential for studies in gene expression, RNA interference, and gene therapy. With its rapid cellular uptake, it is an ideal tool in fields like genetic engineering, personalized medicine.
LNP FluoGreen mRNA Delivery Kit is suitable for transfecting up to 20 µg of mRNA, for a maximum RNA loading capacity of 5 µg per vial, with a recommended lipid nanoparticles concentration of 1 µg/ml. If necessary, PROGEN can offer increased loading capacities in the form of a custom formulation. Additionally, customized nanoparticles with surface-decoration of specific ligands for selective targeting can be delivered upon request, as well as tagging of different fluorophores such as Cy3, Cy5, Cy7 for optimal cell tracking or uptake control purposes. Moreover, PROGEN can also provide solutions for co-encapsulation of different nucleic acids (e.g., for CRISPR/Cas9 systems).
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