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LNP Small Molecule Delivery Kit

Key Features
  • Easy to use
  • High loading capacity
  • Provides robust protection against degradation
  • High stability in diverse biological media
  • Narrow size distribution
  • Facilitates biodistribution, enhancing pharmacokinetics and overall drug efficacy
  • Minimal cytotoxicity in vitro and in vivo
  • Available with fluorescence option, facilitating real-time tracking of drug-loaded nanoparticles in vitro
Product description
Quantity 1 kit
Storage before reconstitution -20ºC (avoid freeze/thaw cycles)
Storage after reconstitution Up to 48 hours at 2-8 ºC
Intended use Research use only
Reconstitution Formulate in Ethanol 96% per protocol.
Application Nanoparticle reagent kit for small molecule delivery
Specificity Small molecules
Stability

Do not freeze Small Molecule Delivery LNPs.

Do not heat up Small Molecule Delivery LNPs.

 

Synonym

LNP

Background

Nanoparticle reagent kit for small molecule delivery

Lipid Nanoparticle reagent kit for enhancing the solubility of hydrophobic and amphiphilic drugs, enabling their delivery in vitro and in vivo.

Applications

In vitro

  • Labeling of live cells
  • Intracellular delivery of therapeutic compounds
  • Therapeutic evaluation
  • Cytotoxicity studies

In vivo

  • Biodistribution evaluation
  • Therapeutic evaluation

About the LNP Small Molecule Delivery Kit

PROGENs LNP Small Molecule Delivery Lipid nanoparticles come as a dryed lipid emulsion, easy to formulate by bench-top addition of ethanol.


The Small Molecule Delivery LNPs prevent loaded drugs from external degradation and rapid clearance. They promote optimal biodistribution, high intracellular absorption and low toxicity, boosting their therapeutic effect. Moreover, the Small Molecule Delivery LNPs have shown efficacy in rodents and zebrafish models across various administration methods.


The composition and molar ratios of PROGENs LNP Small Molecule Delivery Kit is optimized for associating and delivering hydrophobic and amphiphilic small molecules and can be used to efficiently deliver the drug of interest to specific cell lines and animal models. Different formulation protocols should be followed based on the nature of your molecule of interest.


PROGENs LNP Small Molecule Delivery Kit has been validated with molecules that possess various molecular weights (from 297 to 974 g/mol), and n-octanol/water partition coefficient (Log POW from –0.4 to 8.5). If your molecule exceeds the mentioned properties and conditions, please enquire for a customized solution. Additionally, PROGEN offers customized solutions for surface-decorated nanoparticles with tissue-specific ligands, enabling targeted delivery.

 

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Category Kit manual
Size 274.33 KB
Filetype pdf
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FAQs
Yes, if necessary, the formulation can be filtered using 0.22 µm filters of PES membrane.
The solvent of preference is EtOH, but you can use any of the solvents listed at Table 1 of the protocol (for using the whole lipid preparation) or Table 2 (for performing optimization tests using 10 μl Aliquots). Alternatively, you can sonicate the lipid preparation vial containing the drug prior addition to the formulation vial.
Yes, please check out the table below for other recommended buffers:

BUFFER SOLUTION
CONCENTRATION
Ultrapure waterN/A
PBS2-50 mM
NaCl150 mM
HEPES10-25 mM
DPBS1X

Contact PROGEN for further technical support.
Optimization steps are outlined in the specific protocol, please, refer to it and check. If you have specific questions, please, contact PROGEN for a customized solution.
Yes, the formulation can be used in vivo (not for use in humans). For specific recommendations or customized solutions, please contact PROGEN.
This will depend on the specific properties of each molecule. Please refer to the specific protocol of each reagent. For in vivo delivery, formulation can be adapted under request to reach specific requirements attending to the dose and route of administration. For small drugs, it can range from 1% to 15% (55 μg - 0,83 mg) with respect to the total amount of lipids (5,5 mg) per vial. We recommend starting with 1%, and then increasing the concentration gradually. For proteins and peptides, it depends on the selected kit and on the specific molecule, but it is possible to load at least 150 ug of protein/peptide per vial.
We have tested in healthy mice to get an insight, but detailed biodistribution should be done with relevant models and with encapsulated drugs / molecules as this affects the biodistribution. We have conducted s.c. (subcutaneous) administration using one or more imaging modalities as we have optimized labeling (PET, SPECT and fluorescent tracking), i.v. (tail vein injection and retroorbital), i.a. (intra-articular), and i.p. (intraperitoneal).
Once formulated, we recommend using the formulation within 24-48 h, according to the provided protocol.
It is not necessary for initial in vitro testing. There is not a big increase in viscosity and the pH from the formulation is suitable for in vitro testing. It Is also possible to use buffers to formulate, as indicated in the kit protocol.
Filtration through 0,45 μm or 0,22 μm will work to eliminate free precipitated compound to obtain the percentage of free compound and then calculate the percentage of encapsulation through the subtraction of the total and free concentrations, in relation to the total concentration. The filtered fraction that has passed through the filter should be analyzed.
Cargo can enter the cell by either endocytosis or passive membrane fusion.
Yes. Formulations are monodisperse with a diameter size around 100 nm.
1 mL of the Small Molecule reagent is enough for 27 delivery tests in 6-well plates, 134 experiments in 24-well plates and 800 experiments in 96-well plates/ Up to 800 tests in 96-well plates /1 mL.

PROGEN LNP Small Molecule Delivery Kit includes 4 vials of 1 mL each, and PROGEN LNP FluoGreen Small Molecule Delivery kit includes 1 vial of 1 mL.

PROGENs LNP Small Molecule Delivery Nanoparticles can be concentrated using an Amicon Ultra Centrifugal Filter. Samples can be concentrated up to 4-fold their original volume (i.e., to a final volume 250 μL).
Diameter size can be measured by Dynamic Light Scattering (DLS) analysis adding to the cuvette 100 μl of formulation with 900 μl of MilliQ water.

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