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LNP mRNA Delivery Kit

Key Features
  • Easy to use
  • Great mRNA transfection efficiency
  • Robust protection against degradation
  • Suitable for 2D and 3D cultures
  • Stable and homogeneous structure, yielding high cell viability
  • Narrow size distribution
  • Effective in vivo without cationic lipids in the emulsion
  • Minimal cytotoxicity in vitro and in vivo
  • Available with fluorescence option, facilitating real-time tracking of mRNA-loaded nanoparticles in vitro.
Product description
Quantity 1 kit
Storage before reconstitution -20ºC (avoid freeze/thaw cycles)
Storage after reconstitution Up to 24 hours at 2-8 ºC
Intended use Research use only
Reconstitution

Formulate in Ethanol 96% and 10 mM Citrate Buffer, pH 3 per protocol.

Application Nanoparticle reagent kit for mRNA delivery
Specificity mRNA
Stability

Do not freeze mRNA Delivery LNPs.

Do not heat up mRNA Delivery LNPs.

 

Synonym

LNP

Background

Nanoparticle reagent kit for nucleic acid delivery

A breakthrough in gene therapy and molecular biology enabling efficient mRNA transfection in cells, including primary cultures and organoids.


Applications

In vitro:

Delivery of different types of oligonucleotides for:

  • Gene addition or replacement: for example, mRNA encapsulation.
  • Gene expression control; e.g. interference studies with siRNA, miRNA, circRNA, and ASOs in living-and fixed cells are possible with customized solutions (available upon request).
  • Gene editing: customized solutions suitable for delivery of CRISPR–Cas9 gene editing or base-editing systems (available upon request).

In vivo:

Delivery of different types of oligonucleotides for:

  • Gene addition or replacement: for example, mRNA encapsulation.
  • Gene expression control; e.g. interference studies with siRNA, miRNA, circRNA, and ASOs in living-and fixed cells are possible with customized solutions (available upon request).
  • Gene editing: customized solutions suitable for delivery of CRISPR–Cas9 gene editing or base-editing systems (available upon request).

Protein expression

Innovative RNA therapeutics and precision medicine research


About the LNP mRNA Delivery Kit:

PROGENs mRNA Delivery LNPs come as a dryed lipid emulsion easy to formulate by bench-top reconstitution with ethanol and citrate buffer for adequate mRNA encapsulation. The formulation can be subjected to pH 7 buffer exchange by ultrafiltration. This kit is a key tool for RNA-based therapeutics: it may be used for delivering different types of oligonucleotides, such as siRNA, miRNA, circRNA, and ASOs.


The high biocompatibility of the LNP mRNA Delivery Kit enables an efficient internalization of the mRNA whilst yielding good cell viabilities in a broad range of mammalian cells, even in difficult-to-transfect cells, including primary cells and organoids. They enable mRNA delivery in living-and fixed cells, which is essential for studies in gene expression, RNA interference, and gene therapy. With its rapid cellular uptake, it is an ideal tool in fields like genetic engineering, personalized medicine.


LNP mRNA Delivery Kit is suitable for transfecting up to 20 µg of mRNA, for a maximum RNA loading capacity of 5 µg per vial, with a recommended lipid nanoparticles concentration of 1 µg/ml. If necessary, PROGEN can offer increased loading capacities in the form of a custom formulation. Additionally, customized nanoparticles with surface-decoration of specific ligands for selective targeting can be delivered upon request, as well as tagging of different fluorophores such as Cy3, Cy5, Cy7 for optimal cell tracking or uptake control purposes. Moreover, PROGEN can also provide solutions for co-encapsulation of different nucleic acids (e.g., for CRISPR/Cas9 systems).

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Category Kit manual
Size 339.54 KB
Filetype pdf
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FAQs
If necessary, the formulation can be concentrated in the buffer exchange step up to 100 µL.
Yes, if necessary, the formulation can be filtered using 0.22 µm filters of PES membrane.
Diameter size can be measured by Dynamic Light Scattering (DLS) analysis.
Yes, the formulation can be used in vivo (not for use in humans). For specific recommendations or customized solutions, please contact PROGEN.
We have tested in healthy mice to get an insight, but detailed biodistribution should be done with relevant models and with encapsulated drugs / molecules as this affects the biodistribution. We have conducted s.c. (subcutaneous) administration using one or more imaging modalities as we have optimized labeling (PET, SPECT and fluorescent tracking), i.v. (tail vein injection and retroorbital), i.a. (intra-articular), and i.p. (intraperitoneal).
Once formulated, we recommend using the formulation within 24-48 h, according to the provided protocol.
It is not necessary for initial in vitro testing. There is not a big increase in viscosity and the pH from the formulation is suitable for in vitro testing. It Is also possible to use buffers to formulate, as indicated in the kit protocol.
Filtration through 0,45 μm or 0,22 μm will work to eliminate free precipitated compound to obtain the percentage of free compound and then calculate the percentage of encapsulation through the subtraction of the total and free concentrations, in relation to the total concentration. The filtered fraction that has passed through the filter should be analyzed.
Cargo can enter the cell by either endocytosis or passive membrane fusion.
Yes. Formulations are monodisperse with a diameter size around 100 nm.
Yes, the nanoparticles can be loaded with any RNA encoding for any protein of interest.
The maximum amount of mRNA to encapsulate is 5 µg per mRNA Prep vial. However, higher amounts can be achieved as a customized formulation. Please, contact PROGEN for a customized solution.
No, but PROGEN can provide customized formulations for other types of nucleic acid.
The number of reactions or wells will depend on the amount of mRNA you wish to add to your assays. Typically, 100 ng of mRNA is added to P96 wells, which yields 200 wells, or 1 µg for P6 wells, which yields 20 wells. This numbers are for the complete kit for 20 µg of mRNA (4 vials x 5 µg each).

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