- Sterile tube in which the LymphoPrep™ is contained below a plastic filter disc
- Highly effective density gradient media for the purification of human mononuclear cells (monocytes and lymphocytes) from diluted blood
Product description
| Quantity | 18 tubes (10 ml) |
|---|---|
| Intended use | Research use only |
| Product description | Lymphoprep ™ is a ready made sterile an endotoxin tested solution for the isolation of human mononuclear cells. The Lymphoprep™ Tube is a sterile plastic tube prefilled with Lymphoprep™. The solution contains sodium diatrizoate and polysaccharide. The Lymphoprep™ Tube consists of two compartments separated by a plastic insert. The lower compartment is prefilled with Lymphoprep™. The plastic insert allows the blood sample to be poured directly into the tube. No layering of the sample is necessary. |
| Density | 1.077 ± 0.001 g/ml |
| Composition | 9.1% (w/v) Sodium Diatrizoate, 5.7% (w/v) Polysaccharide |
| Osmolality | 290 ± 15 mOsm |
| Endotoxin | < 1.0 EU/ml |
| Stability & storage | Lymphoprep™ Tube is produced under sterile conditions. Lymphoprep is stable for 3 years provided the solution is kept sterile and protected from direct sunlight. |
Background
A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Boyum in 1968. For more than 35 years a commercial medium known as LymphoprepTM has been widely used for isolating these cells.
Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than the erythrocytes and the polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an isoosmotic medium with a density of 1.077 g/ml, which allows the erythrocytes and the PMNs to sediment through the medium while retaining the mononuclear cells at the sample/medium interface.
The described method is rapid, simple and reliable and gives excellent results with blood samples from normal individuals and patients.
The success of the standard method for isolation mononuclear cells using Lymphoprep depends to a large extent on the careful layering of the diluted blood sample on top of the centrifugation medium to maintain a sharp interface between the two layers. This procedure requires some practise and can be time consuming with large numbers of samples.
Lymphoprep Tube is a sterile tube in which the Lymphoprep is contained below a plastic filter disc. This allows diluted blood to be poured simply and directly into the tube, the disc preventing any mixing with the separation medium.
Each batch of Lymphoprep is checked on the level of endotoxins using a specific LAL test. The goal is to provide batches with an endotoxin level lower or equal to 0.13 IU/ml. For every batch produced a Certificate of Analysis showing the actual values of density, osmolality and endotoxins is made available upon request. We also claim sterility according to Ph.Eur.
Lymphoprep is manufactured, packed and released in compliance with GMP and ISO 13485.
LymphoprepTM Tube can be used for the preparation of pure lymphocyte suspensions for tissue typing, antilymphocyte sera and immunological research. Thorsby and Brattelie used this technique with only slight modifications in the preparation of pure lymphocyte suspensions for cytotoxicity tests and lymphocyte cultures.
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