OptiPrep™ - Iodixanol

Cat. No: 1893

Available, delivery time 1-7 days

$198.00*

Key Features
  • Highly effective for the separation of full and empty AAV particles
  • Designed for the purification of viruses (such as AAV)
  • Useful to isolate different cell types and organelles
  • Ready-made, sterile and endotoxin-tested solution of iodixanol

Product description
Quantity 1x250 ml
Intended use Research use only
Product description

OptiPrep™ is a ready made, sterile and endotoxin tested solution of Iodixanol, 5,5’-[(2-hydroxy-1-3 propanediyl)-bis(acetylamino)] bis [N,N’- bis(2,3 dihydroxypropyl- 2,4,6-triiodo-1, 3-benzenecarboxamide], designed for the in vitro isolation of biological particles.

Density 1.320 +/- 0.001 g/ml
Composition Iodixanol: 60% (w/v) in water
Osmolality 170 ± 15 mOsm
Endotoxin < 1.0 EU/ml
Stability & storage OptiPrep™ is stable for 3 years provided the solution is kept sterile and protected from light. Prolonged exposure to direct sunlight leads to release of iodine from the molecule. This effect is negligible when working with these solutions on a day to day basis. OptiPrep™ should be stored between 4°C - 24°C. Before use invert the bottles several times to mix the contents.
Background
OptiPrepTM is a sterile endotoxin tested solution of 60% iodixanol in water. Iodixanol was developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing. Iodixanol is non-ionic, non-toxic to cells and metabolically inert. Iodixanol solutions can be made iso-osmotic at all useful densities. Iodixanol solutions have low viscosity and osmolality. Actual endotoxin levels in each batch are usually measured at ≤ 0.13 EU/ml.
 
Applications:
 
1.    Purification of viral particles such as AAV
OptiPrepTM is the ideal solution for virus purification. Using OptiPrepTM gradients, the ratio of infectious particles to total particles is 100 times higher than a virus purified using CsCI gradients.

Benefits of OptiPrepTM for AAV purification:
- Saves time – no need to remove iodixanol for most downstream processes
- Not destructive – causes little or no damage to viral particles
- Greater recovery rate – ten times greater than CsCI gradient alternatives
- Higher infectivity titer –no toxic effects
 
2.    Isolation of full and empty AAV capsids
Decreasing empty capsids in your AAV preparation ensures product safety by minimizing the risk of unwanted immune responses and maximizing transduction efficiency of your AAV gene therapy product.
The key advantages of removing empty capsids:
- Reduce patient risk by minimizing unwanted immune responses
- Maximize your success rate – improved transduction efficiency allows you to use lower titers
- Be ready for clinical trials – focusing on increased safety ensures a smoother transfer
- Manufacture high quality products – by combining with your chromatography manufacturing process
 
3.    Isolation of other biological particles
The high density of OptiPrepTM facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient or through a simple density barrier.
The low viscosity of the isoosmotic gradient (OptiprepTM) provides a rapid and efficient separation as well as an improved resolution of the major cell organelles.
Additionally, the isolation of the following is possible:
- Mammalian and non-mammalian cells
- Subcellular organelles
- Plasma membranes and domains
- Membrane vesicles and cytosol
- Organelles from non-mammalian sources
- Plasma lipoproteins
- Proteins and protein complexes
- Plasmid DNA
- Ribonucleoproteins
 
Protocols and references are available for the isolation of cells, macromolecules, subcellular organelles and membranes and virus purification.

For every batch produced a Certificate of Analysis showing the actual values of density and endotoxin levels is made available upon request. We also claim sterility according to Ph.Eur.

OptiPrepTM is manufactured, packed and released in compliance with GMP and ISO 13485.

Downloads
File
Category
Size
Filetype
Category Protocol
Size 448.87 KB
Filetype pdf
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


Related Products

AAV9 Xpress ELISA
Cat. No: PRAAV9XP
Fast, easy and reliable AAV9 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV9 Titration ELISA
  • ADK9 antibody is used as capture and detection antibody

$837.00*
AAV9 Titration ELISA
Cat. No: PRAAV9
  • ELISA for quantitation of AAV9 capsids
  • Detection of full and empty AAV9 capsids
  • ADK9 antibody is used as capture and detection antibody

$687.00*
AAV2 Xpress ELISA
Cat. No: PRAAV2XP
Fast, easy and reliable AAV2 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV2 Titration ELISAs
  • A20R antibody is used as capture and detection antibody

$837.00*
AAV2 Titration ELISA 2.0R
Cat. No: PRAAV2R
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20R antibody is used as capture and detection antibody

$687.00*
AAV2 Titration ELISA
Cat. No: PRATV
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20 antibody is used as capture and detection antibody

$687.00*
AAV8 Titration ELISA
Cat. No: PRAAV8
  • ELISA for quantitation of AAV8 capsids
  • Detection of full and empty AA8 capsids
  • ADK8 antibody is used as capture and detection antibody

$687.00*
AAV8 Xpress ELISA
Cat. No: PRAAV8XP
Fast, easy and reliable AAV8 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV8 Titration ELISA
  • ADK8 antibody is used as capture and detection antibody

$837.00*
AAV5 Titration ELISA
Cat. No: PRAAV5
  • ELISA for quantitation of AAV5 capsids
  • Detection of full and empty AAV5 capsids
  • ADK5a antibody is used as capture and detection antibody

$687.00*
AAV6 Titration ELISA
Cat. No: PRAAV6
  • ELISA for quantitation of AAV6 capsids
  • Detection of full and empty AAV6 capsids
  • ADK6 antibody is used as capture and detection antibody

$687.00*
AAVrh10 Titration ELISA
Cat. No: PRAAV10
  • ELISA for quantitation of AAVrh10 capsids
  • Detection of full and empty AAVrh10 capsids
  • ADK8 antibody is used as capture and detection antibody

$687.00*