Positive western blot control: anti-Keratin K19 antibody

Cat. No. 64008

Available, delivery time 1-7 days

€70.00*

Key Features
  • Whole cell lysate from MCF7 human breast adenocarcinoma cell line
  • Validated and tested western blot control for: anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), Cat. No. 61010
    anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), Cat. No. 61029
Product description
Quantity 50 µg
Cell line/ tissue MCF7
Application WB
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Species of origin Human
Formulation Lyophilized, reconstitute in 50 µl 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)
Tested positive control for anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), Cat. No. 61010
anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), Cat. No. 61029
Applications
Tested applications Tested dilutions
Western Blot (WB) 5-10 µg total protein per lane
Background

Whole cell lysate from MCF7 human breast adenocarcinoma cell line.

Tested and validated western blot control for:
anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), Cat. No. 61010

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), Cat. No. 61029

MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

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FAQs
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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