Positive western blot control: anti-Vimentin antibody
WB with anti-Vimentin antibody (Cat. No. 61013, 1:500), SV80 whole cell lysate (10 µg) western blot control
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WB with anti-Vimentin antibody (Cat. No. 61013, 1:500), SV80 whole cell lysate (10 µg) western blot control
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Key Features
- Whole cell lysate from SV80 human lung fibroblast (SV40-transformed) cell line
- Validated and tested western blot control for: anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013
Product description
| Quantity | 50 µg |
|---|---|
| Cell line/ tissue | SV80 |
| Application | WB |
| Storage | Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles) |
| Intended use | Research use only |
| Species of origin | Human |
| Formulation | Lyophilized, reconstitute in 50 µl 1 x SDS buffer (lysis buffer composition: PBS + Pefablock) |
| Tested positive control for | anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Western Blot (WB) | 10 µg total protein per lane |
Background
Whole cell lysate from SV80 human lung fibroblast (SV40-transformed) as positive western blot control.
Tested and validated western blot control for:
anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013.
SV80 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.
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FAQs
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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