Key Features
Product description
| Quantity | 250 µg |
|---|---|
| Storage | Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles) |
| Intended use | Research use only |
| Source | Rat, recombinant (his:RAP:c-myc fusion protein) |
| Molecular weight | 40 kDa |
| Purity | > 95% (determined by SDS gelelectrophoresis) |
| Reconstitution | When reconstituted with 250 µl distilled water, solution will contain 1 mg/ml rat RAP protein in TBS, pH 7.5, 0.1% BSA, 0.09% NaN3. |
| Application | Protein standard in 1D and 2D SDS gelelectrophoresis, immunoblotting and receptor-binding studies |
Background
Protein standard for immunoblotting, immunization, immunoassay and receptor-binding studies. Sequence highly homolog with human RAP.
Rat his:RAP:c-myc fusion protein.
Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA (Bajari et al. 2005). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).