Simple Plex AAV2 Assay

Cat. No. SPCKB-OT-005905

Available, delivery time 1-7 days

€798.00*

Key Features
  • Automated ELISA kit for quantification of AAV2 virons and assembled capsids
  • A20R antibody (Cat. No. 610298) is used as capture and detection antibody
  • Suitable for the ProteinSimple Ella system
Product is not available in APAC countries
Product description
Quantity 1x72 tests
Reactivity AAV2
Storage 2-8°C
Intended use Research use only
Application ELISA
Product description Microfluidic Simple Plex assay cartridge for use with the Ella platform. The Simple Plex AAV2 assay utilizes PROGEN's A20R antibody (Cat. No. 610298) to deliver proven specificity with a fast and convenient automated workflow.
Form supplied Reagent kit, 1x72 wells
Background
The Simple Plex AAV2 assay is a sandwich style immunoassay carried out within the microfluidic circuitry of a Simple Plex cartridge. Capture antibody coated glass nano-reactors (GNRs), biotinylated detection antibody, and streptavidin dye conjugate are all assembled within the cartridge. Users simply load their diluted samples and wash buffer into the cartridge before placing it in the Ella instrument. Ella then automates every step of the assay process from wash steps to final quantification of results in just 90 minutes.

The ELISA principle:

The assay is based on the sandwich ELISA technique where a recombinant antibody specific for a conformational epitope on assembled AAV capsids is coated onto the plate and is used to capture AAV particles from the specimen.

The detection of captured AAV particles is a two-step process.
1. A biotin-conjugated recombinant antibody is bound to the captured AAV particles.
2. A streptavidin peroxidase conjugate reacts with the biotin molecules. The addition of the substrate results in a color reaction which is proportional to the amount of specifically bound viral particles.

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Size
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Category Kit manual
Size 479.92 KB
Filetype pdf
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