Vimentin, bovine, 250 µg

Cat. No. 62011

Available, delivery time 1-7 days

€554.00*

Key Features
  • Purified bovine vimentin
  • Protein standard
Product description
Quantity 250 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Bovine lens
Molecular weight 57 kDa
Isoeletric point pI 5.3
Purity > 98% (determined by SDS gelelectrophoresis)
Reconstitution Reconstitute with 200 µl distilled water (final volume 250 µl).
Final solution: 10 mM sodium phosphate, pH 7.5, 2 mM DTT, 6 M urea, 10 mM methylammonium chloride, 1 mM EDTA;
Protein concentration: 1 mg/ml (according to Bradford)
Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Background
Protein standard for immunoblotting, immunization and immunoassay.
Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM sodium phosphate, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
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