Call +49 6221 8278-0

PROGEN - passion for research

close×

WB with anti-Keratin K13 antibody (Cat. No. 61007, 1:50), MCF7 whole cell lysate (5-10 µg) western blot control Zoom

WB with anti-Keratin K13 antibody (Cat. No. 61007, 1:50), MCF7 whole cell lysate (5-10 µg) western blot control

New

AAV8 ELISA Control

The AAV8 ELISA Control consists of fully assembled, empty AAV8 capsids. It represents the serotype-specific conformational epitopes and is therefore a reliable positive control in detection and quantification assays based on AAV antibodies detecting the conformational epitopes, such as ELISA.
Cat. No.: PRAAV8-C
Quantity:  1 vial (2.8E+08 – 4.7E+08 capsids)

Delivery Time: usually 1-7 working days

$135.00
Excl. VAT, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
PRAAV1-C

AAV1 ELISA Control

1 vial (0.6E+09 – 1.0E+09 capsids)
$135.00
Excl. VAT, excl. Shipping Cost
PRAAV2-C

AAV2 ELISA Control

1 vial (0.8E+09 – 1.4E+09 capsids)
$135.00
Excl. VAT, excl. Shipping Cost
PRAAV3-C

AAV3 ELISA Control

1 vial (5.5E+08 – 9.5E+08 capsids)
$135.00
Excl. VAT, excl. Shipping Cost
PRAAV5-C

AAV5 ELISA Control

1 vial (3.9E+09 – 6.5E+09 capsids)
$135.00
Excl. VAT, excl. Shipping Cost
PRAAV6-C

AAV6 ELISA Control

1 vial (1.0E+09 – 1.6E+09 capsids)
$135.00
Excl. VAT, excl. Shipping Cost
PRAAV9-C

AAV9 ELISA Control

1 vial (4.5E+08 – 7.5E+08 capsids)
$135.00
Excl. VAT, excl. Shipping Cost

Product description

Formulation Lyophilized, empty AAV8 capsids. Reconstitute in 750 μl ASSB 1x (provided with PROGEN’s AAV8 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer.
Storage store at 2-8°C
Concentration

3.8E+08 – 6.3E+08 capsids/ml after reconstitution in 750 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial)

Intended use Research use only
Stability 4 weeks at 2-8°C after reconstitution in ASSB 1x

Applications

Tested applications Tested dilutions
ELISA As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN’s AAV8 Titration ELISA) and analysis at least in duplicates is recommended.

Background

Details

The AAV8 ELISA Control consists of fully assembled, empty AAV8 capsids. The concentration is lot specific.

The AAV8 ELISA Control can be used as a positive control with PROGEN’s AAV8 Titration ELISA (PRAAV8). If you require different lots of the AAV8 ELISA Control and the Kit Control included in your PROGEN AAV8 Titration ELISA, please enquire to check availability.

Reading of the AAV8 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN’s AAV8 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.

The AAV8 ELISA Control has been calibrated on the ATCC AAV8 reference standard material (RSM, VR-1816). For further information, please see the publication by Ayuso et al., 2014.

Publications

Ayuso, E. et al. Manufacturing and characterization of a recombinant adeno-associated virus type 8 reference standard material. Hum. Gene Ther. 25, 977–987 (2014).

D’Costa, S. et al. Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR. Mol. Ther. Methods Clin. Dev. 5, 16019 (2016).

Gurda, B. L. et al. Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8. J. Virol. 86, 7739–7751 (2012).

Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686–12697 (2011).

 

Reference

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields