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Acidic Fibroblast Growth Factor, human, 1 mg

Growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells. Half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells) is reached using < 3 ng/ml.
Cat. No.: 63110
Quantity:  1 mg

Delivery Time: usually 1-7 working days

€3,090.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Purity > 95%
Source human recombinant, produced in E. coli

Background

Details

Molecular Weight
20 000

Purification
Heparin affinity chromatography

Biological Activity
≤ 3 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells).

Reconstitution
Reconstitute in 100 μl (10 μg; 25 μg) or 1 ml (1 mg) dist. H2O, dilute further in PBS as required.

Storage
at 2-8°C (lyoph.)
at -20°C (reconstituted)

Reference

Reference

Thomas KA et al: Purification and characterization of acidic fibroblast growth factor from bovine brain. Proc Natl Acad Sci USA 81, 357- 361 (1984)

Böhlen P et al: Acidic fibroblast growth factor (FGF) from bovine brain; amino-terminal sequence and comparison with basic FGF. EMBO J 4, 1951-1965 (1985)

Gimenez-Gallego G et al: Brain-derived acidic fibroblast growth factor: Complete amino acid sequence and homologies. Science 230, 1385-1388 (1985)

Jaye M et al: Human endothelial cell growth factor: Cloning, nucleotide sequence and chromosome localization. Science 233, 541-545 (1986)

Mergia A et al: The genes for basic and acidic fibroblast growth factors are on different human chromosomes. Biochem Biophys Res Comm 138, 644-651 (1986)

Linemeyer D et al: Expression in E. coli of a chemically synthesized gene for biologically active, bovine acidic fibroblast growth factor. Biotechnology 5, 960-964 (1987)

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