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alpha-Actinin, chicken

Protein standard for immunoblotting, immunization and immunoassays.
Cat. No.: 62002
Quantity:  250 µg

Delivery Time: usually 1-7 working days

€297.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Purity > 95%
Source chicken gizzard

Background

Details

Molecular Weight
95,000

Application
Protein standard in 1D and 2D SDS gelelectrophoresis, Immunoassays, Immunization

Isoelectric Point
pI 5.8

Storage
At 2-8°C (lyoph.); at -20°C (reconstituted)

Reconstitution
Reconstitute with 250 μl distilled water (final volume 250 μl), Final solution: 10 mM Tris/acetate buffer pH 7.6, 0.1mM EDTA, 2 mM DTT, 20 mM NaCl; protein concentration: 1 mg/ml (according to Bradford)

Reference

Reference

Duhaiman AS and Bamburg JR. Isolation of brain alpha-actinin. Its characterizationm and a comparison of its properties with those of muscle alpha-actinins. Biochemistry 23, 1600 ff (1984)

Feramisco JR and Burridge K: A rapid purification of a-actinin, filamin and a 130,000 dalton protein from smooth muscle. J Biol Chem 255, 1194ff (1980)

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