Epitope mapped
Frequently used
Linear epitope

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDye™ 488 Conjugate

Liquid

Supernatant

AFDye™ 488

AFDye™ 647

Liquid

Cat. No. 61058-488

Available, delivery time 1-7 days

€498.00*

Key Features
  • AFDye™ 488 conjugate
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1
Product description
Quantity 50 µg (100 µl)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate AFDyeTM 488
Application WB
Purification Affinity chromatography
Reactivity AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVDJ, AAVrh10
Storage Up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone B1
Immunogen AAV2 capsids
Concentration 500 µg/ml
Formulation Liquid; PBS + 0.09% sodium azide
Applications
Tested applications Tested dilutions
Western Blot (WB) 1:2,500-1:5,000 (0.1-0.2 µg/ml; detection limit 5E+09 capsids)
Background
The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

The AFDyTM 488 is a green emitting dye, structurally and spectrally equivalent to Alexa FluorR 488 and therefore ideally suited for the 488 nm laser line (excitation/emission 494/517 nm). It can be used with a common FITC filter set.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Alexa FluorR is a registered trademark of Thermo Fisher Scientific.

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (23)
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Category Protocol
Size 919.99 KB
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FAQs

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

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