Dot blot analysis of denatured and native AAV1-AAV9, AAVrh10, AAVDJ, AAVrh74 capsids (1E+09-1E+10 capsids) using the anti-AAV VP1/VP2/VP3 rabbit polyclonal antibody VP51. Capsids were denatured at 95°C for 10 min in sample buffer.
The nitrocellulose membrane was blocked with 5% milk in PBST for 1 h at RT. The primary antibody anti-AAV VP1/VP2/VP3 rabbit polyclonal, VP51 (Cat. No. 61084) was diluted in blocking buffer (1:250) and incubated for 1 h at RT. The secondary antibody anti-rabbit IgG goat polyclonal, HRP conjugate (Cat. No. 90003) was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Species/Reactant: Homo sapiens (Human)
Applications: Immunocytochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Species/Reactant: Homo sapiens (Human)
Applications: Immunocytochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Dot blot analysis of denatured and native AAV1-AAV9, AAVrh10, AAVDJ, AAVrh74 capsids (1E+09-1E+10 capsids) using the anti-AAV VP1/VP2/VP3 rabbit polyclonal antibody VP51. Capsids were denatured at 95°C for 10 min in sample buffer.
The nitrocellulose membrane was blocked with 5% milk in PBST for 1 h at RT. The primary antibody anti-AAV VP1/VP2/VP3 rabbit polyclonal, VP51 (Cat. No. 61084) was diluted in blocking buffer (1:250) and incubated for 1 h at RT. The secondary antibody anti-rabbit IgG goat polyclonal, HRP conjugate (Cat. No. 90003) was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Species/Reactant: Homo sapiens (Human)
Applications: Immunocytochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Species/Reactant: Homo sapiens (Human)
Applications: Immunocytochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
- Rabbit polyclonal
- Suitable for ELISA, ICC/IF, IP and WB
- Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
- Strong affinity for denatured capsid proteins
- Weak affinity for native capsids
Product description
| Quantity | 250 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Application | ELISA, ICC/IF, IP, WB |
| Purification | Stabilized antiserum |
| Reactivity | AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVDJ, AAVrh10, AAVrh74 |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Recombinant AAV2 VP3 proteins |
| Formulation | Contains 0.09% sodium azide |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:50-1:100 |
| ELISA | Assay dependent |
| Immunoprecipitation (IP) | 1:10 |
| Western Blot (WB) | 1:200-1:500 |
Background
References/Publications (10)
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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