Conformational epitope
Neutralizing
Recombinant

anti-AAV1, human chimeric, ADK1a-h1

Liquid

Liquid

Cat. No. 692350

Available, delivery time 1-7 days

€450.00*

Key Features
  • Recombinant chimeric (human Fc region)
  • Suitable for dot blot, neutralization assay and serology ELISA
  • Reacts with AAV1 and AAV6 intact capsids
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Recombinant chimeric (human Fc region)
Isotype IgG1
Conjugate Unconjugated
Application Dot blot, Neutralization assay, Serology ELISA
Purification Affinity chromatography
Reactivity AAV1, AAV6
No reactivity AAV2, AAV3, AAV4, AAV5, AAV8, AAV9, AAVDJ, AAVrh10, AAVrh74
Storage 2-8°C
Intended use Research use only
Clone ADK1a-h1
Immunogen AAV1 capsids
Concentration

50 µg/ml

Formulation PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
Binding affinity

KD value (AAV1) = 8.8E-12 M

KD value (AAV6) = 5.9E-11 M

Synonym Adeno-associated virus 1; AAV-1
Applications
Tested applications Tested dilutions
Serology Assay 0.1-0.4 µg/ml (1:125-1:500; AAV6 ELISA)
Dot Blot 0.1 µg/ml (1:500; non-denaturing conditions)
Neutralization Assay EC50 ~1 ng/ml (AAV1 and AAV6) - assay dependent
Background

Our human chimeric AAV antibodies are derived from our mouse monoclonal AAV antibodies and are a combination of the mouse antigen binding region and a human Fc region. Therefore, it provides the well-known characteristics of the corresponding mouse monoclonal antibody (anti-AAV1, ADK1a, Cat. No. 610150) like binding affinity, cross-reactivity and neutralization activity combined with a human Fc region allowing the use in an anti-human secondary antibody detection system.

Many humans in the general population have developed antibodies against AAV as a result of naturally acquired infections, which might affect efficacy and safety of the gene transfer using AAV vectors. Therefore, testing for pre-existing AAV antibodies in patient sera is an indispensable step for the selection of patients for AAV gene therapy clinical trials.

Our human chimeric AAV antibodies are close to the human derived samples, making them the ideal positive control for reproducible and comparable serological assays.

Limited Use Label License: Research Use Only
Product is exclusively owned to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

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