Conformational epitope
Frequently used
Neutralizing

anti-AAV5 (intact particle) mouse monoclonal, ADK5b, lyophilized, purified

Lyophilized

Lyophilized

Cat. No. 610149

Available, delivery time 1-7 days

€358.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC and neutralization assays
  • Reacts with AAV5 intact capsids
  • Isotype: IgG2b kappa
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b kappa
Conjugate Unconjugated
Application Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification Affinity chromatography
Reactivity AAV5
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ADK5b
Immunogen AAV5 capsids
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym Adeno-associated Virus 5, AAV-5
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Dot Blot 1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA Assay dependent
Immunoprecipitation (IP) 1:5
Neutralization Assay Assay dependent
Background

For characterization of different stages of infection and very useful for the analysis of the AAV5 assembly process. ADK5b specifically reacts with intact adeno-associated virus 5 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
The antibody is usefull for neutralizing experiments.

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (3)
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FAQs

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

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