Conformational epitope
Neutralizing
Recombinant

anti-AAV8, human chimeric, ADK8-h1

Liquid

Liquid

Cat. No. 692318

Available, delivery time 1-7 days

€450.00*

Key Features
  • Recombinant chimeric (human Fc region)
  • Suitable for dot blot, neutralization assay and serology ELISA
  • Reacts with AAV3, AAV7, AAV8, AAVrh10 and AAVrh74 intact capsids
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Recombinant chimeric (human Fc region)
Isotype IgG1
Conjugate Unconjugated
Application Dot blot, Neutralization assay, Serology ELISA
Purification Affinity chromatography
Reactivity AAV3, AAV7, AAV8, AAVrh10, AAVrh74
No reactivity AAV1, AAV2, AAV4, AAV5, AAV6, AAV9, AAVDJ
Storage 2-8°C
Intended use Research use only
Clone ADK8-h1
Immunogen AAV8 capsids
Concentration

50 µg/ml

Formulation PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
Binding affinity

KD value (AAV8) = <1.0E-12 M

KD value (AAVrh10) = <1.0E-12 M

KD value (AAVrh74) = <1.0E-12 M

Synonym

Adeno-associated virus 8; AAV-8

Applications
Tested applications Tested dilutions
Serology Assay 0.1-0.4 µg/ml (1:125-1:500; AAV8 ELISA)
Dot Blot 0.1 µg/ml (1:500; non-denaturing conditions)
Neutralization Assay EC50 ~26 ng/ml (AAV3), ~2 ng/ml (AAV8) - assay dependent
Background

Our human chimeric AAV antibodies are derived from our mouse monoclonal AAV antibodies and are a combination of the mouse antigen binding region and a human Fc region. Therefore, it provides the well-known characteristics of the corresponding mouse monoclonal antibody (anti-AAV8, ADK8, Cat. No. 610160) like cross-reactivity and neutralization activity combined with a human Fc region allowing the use in an anti-human secondary antibody detection system.

Many humans in the general population have developed antibodies against AAV as a result of naturally acquired infections, which might affect efficacy and safety of the gene transfer using AAV vectors. Therefore, testing for pre-existing AAV antibodies in patient sera is an indispensable step for the selection of patients for AAV gene therapy clinical trials.

Our human chimeric AAV antibodies are close to the human derived samples, making them the ideal positive control for reproducible and comparable serological assays.

Limited Use Label License: Research Use Only
Product is exclusively owned to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

Downloads
File
Category
Size
Filetype
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


FAQs

The human chimeric AAV antibodies have the same variable region as our mouse monoclonal antibodies but instead a human constant region. 

Yes, they show the same cross-reactivity as their mouse monoclonal versions. The table below shows where cross-reactivity takes place.

Human Chimeric Antibody Clone AAV1 AAV2 AAV3 AAV4 AAV5 AAV6 AAV7 AAV8 AAV9 AAVrh10 AAVrh74 AAVDJ
Anti-AAV1 ADK1a-h1 X         X n.a.*          
Anti-AAV2 A20-h1   X X       n.a.*          
Anti-AAV5 ADK5b-h1         X   n.a.*          
Anti-AAV5 ADK5b-h2         X   n.a.*          
Anti-AAV8 ADK8-h1     X       X X   X X  
Anti-AAV9 ADK9-h1             n.a.*   X      

 

*n.a.: not analyzed

The binding affinity of the human chimeric antibodies to different AAV serotypes was tested and compared to the corresponding mouse monoclonal antibodies using biolayer interferometry (BLI) analysis. The table below shows the results. 

Serotype Antibody Clone Binding Affinity
(KD Value)
AAV1 ADK1a <1.0E-12 M
ADK1a-h1 8.8E-12 M
     
AAV2 A20 2.6E-11 M
A20-h1 1.2E-10 M
A20R <1.0E-12 M
     
AAV3 A20 <1.0E-12 M
A20-h1 9.3E-11 M
A20R <1.0E-12 M
     
AAV5 ADK5a 4.9E-11 M
ADK5b 6.0E-11 M
ADK5b-h1 8.1E-11 M
ADK5b-h2 3.6E-11 M
     
AAV6 ADK1 <1.0E-12 M
ADK1a-h1 5.9E-11 M
     
AAV8 ADK8 <1.0E-12 M
ADK8-h1 <1.0E-12 M
     
AAV9 ADK9-1R <1.0E-12 M
ADK9-h1 7.0E-11 M
     
AAVrh10 ADK8 <1.0E-12 M
ADK8-h1 <1.0E-12 M
     
AAVrh74 ADK8 <1.0E-12 M
ADK8-h1 <1.0E-12 M


The lower the KD value the higher the binding affinity. The detection limit is 1.0E-12 M.

Related Products

AAV8 ELISA Control
Cat. No. PRAAV8-C
  • Consists of fully assembled, empty AAV8 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

€110.00*
AAV8 empty capsids
Cat. No. 66V080
  • Fully assembled, empty AAV8 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*
AAV8 standard material (eGFP)
Cat. No. 66V081
  • Fully assembled AAV8 capsids filled with an eGFP reporter gene controlled by a CMV promoter
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
  • Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
Not available in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€580.00*
AAV8 Titration ELISA
Cat. No. PRAAV8
  • ELISA for quantitation of AAV8 capsids
  • Detection of full and empty AA8 capsids
  • ADK8 antibody is used as capture and detection antibody

€560.00*
AAV8 Xpress ELISA
Cat. No. PRAAV8XP
Fast, easy and reliable AAV8 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV8 Titration ELISA
  • ADK8 antibody is used as capture and detection antibody

€685.00*
AAVrh10 Titration ELISA
Cat. No. PRAAV10
  • ELISA for quantitation of AAVrh10 capsids
  • Detection of full and empty AAVrh10 capsids
  • ADK8 antibody is used as capture and detection antibody

€560.00*
anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
Cat. No. 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

€382.00*
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No. 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with denatured VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1

Variants from €110.00*
€382.00*
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No. 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
  • Strong affinity for denatured capsid proteins
  • Weak affinity for native capsids

€382.00*
anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purified
Cat. No. 610160
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization
  • Reacts with AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa

Variants from €110.00*
€363.00*
anti-AAV8, human chimeric, ADK8-h1
Cat. No. 692318
Quantity: 1 ml (50 µg/ml)
  • Recombinant chimeric (human Fc region)
  • Suitable for dot blot, neutralization assay and serology ELISA
  • Reacts with AAV3, AAV7, AAV8, AAVrh10 and AAVrh74 intact capsids
  • Isotype: IgG1

Variants from €170.00*
€450.00*
anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9, liquid, purified
Cat. No. 690161
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for dot blot and ICC/IF
  • Reacts with AAV8, AAVrh10, Anc80 and AAVrh74 intact particles
  • Weak affinity for AAV9 and AAVDJ intact particles
  • Isotype: IgG2a kappa

Variants from €110.00*
€363.00*
Dip’n’Check AAV8 - lateral flow assay
Cat. No. PR5208
  • Rapid lateral flow test for the detection of AAV8 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV8 capsids

€163.00*
OptiPrep™ - Iodixanol
Cat. No. 1893
  • Highly effective density gradient media for the separation of full and empty AAV particles
  • Designed for the purification of viruses (such as AAV)
  • Useful to isolate different cell types and organelles
  • Ready-made, sterile and endotoxin-tested solution of iodixanol

 

€178.00*
Simple Plex AAV8 Assay
Cat. No. SPCKB-OT-007970
  • Automated ELISA kit for quantification of AAV8 virons and assembled capsids
  • ADK8 antibody (Cat. No. 610160) is used as capture and detection antibody
  • Suitable for the ProteinSimple Ella system
Product is not available in APAC countries

€798.00*