Conformational epitope
Frequently used
Neutralizing

anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant

Lyophilized

Lyophilized

Supernatant

Biotin

Cat. No. 651160

Available, delivery time 1-7 days

€253.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for dot blot, ELISA and neutralization
  • Reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
  • Isotype: IgG2a kappa
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification Hybridoma cell culture supernatant
Reactivity AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74, Anc80
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ADK8
Immunogen AAV8 capsids
Formulation Contains 0.09% sodium azide
Synonym Adeno-associated virus 8, AAV-8
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Dot Blot Assay dependent (non-denaturing conditions)
ELISA Assay dependent
Immunoprecipitation (IP) Assay dependent
Neutralization Assay Assay dependent
Background

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK8 specifically reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Predicted binding site: residues 586-LQQQNT-591 (Gurda et al. 2012). The antibody cannot be used for immunoblotting using denaturing conditions.
The antibody is also useful for neutralizing experiments.
Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739-7751 (2012).

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (5)
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FAQs

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

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