Conformational epitope
Neutralizing
Recombinant

anti-AAV9 (intact particle) mouse recombinant, ADK9-1R, lyophilized, purified

Lyophilized

Lyophilized

Cat. No. 610178

Available, delivery time 1-7 days

€440.00*

Key Features
  • Purified, lyophilized
  • Recombinant antibody
  • Suitable for dot blot, ELISA and neutralization assay
  • Reacts with AAV9 intacts particles
  • Isotype: mouse IgG1
Product description
Quantity 50 µg
Antibody Type Recombinant
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application Dot blot, ELISA, Neutralization assay
Purification Affinity chromatography
Reactivity AAV9
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ADK9-1R
Immunogen AAV9 capsids
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym Adeno-associated virus 9; AAV-9
Applications
Tested applications Tested dilutions
Dot Blot 1:500 (0.1 µg/ml, non-denaturing conditions)
ELISA Assay dependent
Neutralization Assay Assay dependent
Background

For characterization of different stages of infection and very useful for the analysis of the AAV9 assembly process. ADK9-1R specifically reacts with intact AAV9 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
The antibody is also useful for neutralizing experiments.
The ADK9-1R antibody recognizes the same epitope as the ADK9 antibody (Cat. No. 690162).

Limited Use Label License: Research Use Only
Product is exclusively owned by PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

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FAQs
ADK9 is a mouse monoclonal IgA antibody, while ADK9-1R is a recombinant mouse IgG1 antibody. Both antibodies recongize the same epitope, only the antibody backbone is different.
There is no difference in performance.

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

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