Disease related

anti-ACTH mouse monoclonal, 2F6, purified

Cat. No. 691513

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF and IHC
  • Reacs with rat
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Rat
Storage 2-8°C
Intended use Research use only
Clone 2F6
Immunogen ACTH synthetic peptide (aa 1-24) conjugated to KLH
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P01194 (Rat)
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:25-1:50 (2-4 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:50 (2-4 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

ACTH (same as corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. 2F6 is specific to synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH). ACTH is also produced by cells of the immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress.

Positive control: pituitary gland, pituitary tumor.

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