Diagnostic marker

anti-Aflatoxin B1 and B2 mouse monoclonal, AFT14 (CBL03), purified

Cat. No. 691534

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA and WB
  • Reacts with Aspergillus
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application ELISA, WB
Purification Affinity chromatography
Reactivity Aspergillus
Storage 2-8°C
Intended use Research use only
Clone AFT14 (CBL03)
Immunogen Aflatoxin obtained from Aspergillus
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
ELISA Assay dependent
Western Blot (WB) 1:1-1:2 (50-100 µg/ml)
Background

AFT14 reacts with aflatoxin B1 and B2, a 55 kDa protein secreted by Aspergillus. The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450-dependent) in the tissues (in particular the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA, and protein. Cytochrome p450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1 molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione S-transferase) and its subsequent excretion is regarded as an important detoxification pathway in animals. Aflatoxins are well recognized as a cause of liver cancer, but they have additional important toxic effects. Aflatoxin B1 is a potent hepatocarcinogenic and mutagenic mycotoxin of Aspergillus flavus.

Positive control: aflatoxin B1 and B2 of Aspergillus flavus.

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