anti-Butyrophilin mouse monoclonal, 102.43, supernatant
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with bovine and human
- Isotype: IgG2a kappa
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Butyrophilin purified from bovine milk fat globule membrane|
|Formulation||Contains 0.09% sodium azide|
|UniprotID||P18892 (Bovine),Q13410 (Human)|
|Synonym||Butyrophilin subfamily 1 member A1, BT, BTN1A1, BTN|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||Ready-to-use|
|Western Blot (WB)||Assay dependent|
The mab reacts specifically with butyrophilin, a unique 67 kD polypeptide present in lactating mammary gland epithelium and in the milk fat globule membrane (MFGM).
No cross-reaction with other MFGM proteins. The epitope has been localized on the N-terminal extracellular part of the protein, close to the transmembrane region.
Butyrophilins have recently been described as a new family of immunoregulators, similar to the co-stimulatory and co-inhibitory family of B7 molecules (Arnett & Viney, 2014).
Polypeptide reacting:Butyrophilin, 67 kD polypeptide; present in the apical membrane of lactating mammary epithelium and in MFGM.
Reactivity on cultured cell lines: Transfected cell line BMGE with butyrophilin cDNA.
Positive control: Milk fat globule membrane (MFGM).
Arnett HA & Viney JL: Immune modulation by butyrophilins. Nature Review Immunology 14, 559-569 (2014)
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