anti-CD100 mouse monoclonal, EBS-CD-045, purified

Cat. No. 691612

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human, monkey and mouse
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human, Monkey, Mouse
Storage 2-8°C
Intended use Research use only
Clone EBS-CD-045
Immunogen PHA stimulated PBL
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID Q92854 (Human), O09126 (Mouse)
Synonym Semaphorin-4D, A8, BB18, GR3, CD antigen CD100, SEMA4D, C9orf164, CD100, SEMAJ
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

EBS-CD-045 reacts with human CD100, a 150 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T-cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK-cells, EBV transformed B-cells, monocytes and tumor T-cell lines. It plays a role in homotypic cell adhesion and in T-cell activation.

Positive control: Daudi, Raji, HUT-78, Kg1a and U937 cells, PBL, tonsil and lymph node.

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