anti-CD15/FUTU4/Lex mouse monoclonal, Bra4F1, purified

Cat. No. 691538

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF and IHC
  • Reacts with human
  • Isotype: IgM kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgM kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone Bra4F1
Immunogen k562 (erythroid-myeloid leukemia cell line)
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P22083 (Human)
Synonym Alpha-(1,3-fucosyltransferase 4, EC 2.4.1.-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

Bra4F1 reacts with CD15 (220 kDa). CD15 is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is further expressed in Reed-Sternberg cells in classic Hodgkin's disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Bra4F1 was clustered at the IVth International Workshop on Leucocyte Differentiation Antigens.

Positive control: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

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