anti-CD16 mouse monoclonal, ICO-116, purified

Cat. No. 691563

Available, delivery time 1-7 days

€340.00*

Key Features
  • mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone ICO-116
Immunogen Human PBL
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

ICO-116 specifically recognizes CD16. This molecule also named low affinity Fc receptor for IgG (FcgammaRIII) exhibits two truncated Ig-like domains and is 50-80 kDa. It is highly expressed on NK-cells, granulocytes and macrophages. ICO-116 binds to 15% peripheral lymphocytes of healthy donors (NK-cells), granulocytes, macrophages. CD16 represents the functional receptor structure for antibody-dependent cellular cytotoxicity.

Positive control: K-562 or U-937 cells. Lymph nodes and tonsils.

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