anti-CD176 mouse monoclonal, EBS-CD-054, purified

Cat. No. 691617

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human
  • Isotype: IgM kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgM kappa
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone EBS-CD-054
Immunogen Neuraminidase-treated human red blood cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml)
ELISA Assay dependent
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)

EBS-CD-054 recognizes the carbohydrate epitope Thomsen-Friedenreich (asialo-glycophorin; Gal-beta1-3GalNAc). It is specific for both anomeric forms of this disaccharide (TF-alpha and TF-beta, including related structures on glycolipids). Immunoblotting with human acute myelogenous leukemia cells revealed a series of TF-active glycoproteins with a main band at about 155 kDa. It shows no cross-reactivity with sialylated glycophorin. Human cells normally do not carry Thomsen-Friedenreich antigen. Only after malignant transformation the antigen may appear and is thus considered a pan-carcinoma marker. EBS-CD-054 was typed at the 7th CD-Workshop.

Positive control: KG1 cells or human colorectal carcinoma tissues.

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